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The Tri-phasic Role of Hydrogen Peroxide in Blood-Brain Barrier Endothelial cells.

ABSTRACT: Hydrogen peroxide (H2O2) plays an important role physiologically as the second messenger and pathologically as an inducer of oxidative stress in injury, ischemia and other conditions. However, it is unclear how H2O2 influences various cellular functions in health and disease differentially, particularly in the blood-brain barrier (BBB). We hypothesized that the change in cellular concentrations of H2O2 is a major contributor in regulation of angiogenesis, barrier integrity/permeability and cell death/apoptosis in BBB endothelial cells. Rat brain microvascular endothelial cells were exposed to various concentrations of H2O2 (1?nM to 25?mM). BBB tight junction protein (zonula ocludens-1; ZO-1) localization and expression, cytoskeletal organization, monolayer permeability, angiogenesis, cell viability and apoptosis were evaluated. H2O2 at low concentrations (0.001??M to 1??M) increased endothelial cell tube formation indicating enhanced angiogenesis. H2O2 at 100??M and above induced monolayer hyperpermeability significantly (p?

SUBMITTER: Anasooya Shaji C 

PROVIDER: S-EPMC6333800 | biostudies-other | 2019 Jan

REPOSITORIES: biostudies-other

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The Tri-phasic Role of Hydrogen Peroxide in Blood-Brain Barrier Endothelial cells.

Anasooya Shaji Chinchusha C   Robinson Bobby D BD   Yeager Antonia A   Beeram Madhava R MR   Davis Matthew L ML   Isbell Claire L CL   Huang Jason H JH   Tharakan Binu B  

Scientific reports 20190115 1

Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays an important role physiologically as the second messenger and pathologically as an inducer of oxidative stress in injury, ischemia and other conditions. However, it is unclear how H<sub>2</sub>O<sub>2</sub> influences various cellular functions in health and disease differentially, particularly in the blood-brain barrier (BBB). We hypothesized that the change in cellular concentrations of H<sub>2</sub>O<sub>2</sub> is a major contributor in re  ...[more]

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