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Characterizing gibberellin flow in planta using photocaged gibberellins.


ABSTRACT: Gibberellins (GAs) are ubiquitous plant hormones that coordinate central developmental and adaptive growth processes in plants. Accurate movement of GAs throughout the plant from their sources to their destination sites is emerging to be a highly regulated and directed process. We report on the development of novel photocaged gibberellins that, in combination with a genetically encoded GA-response marker, provide a unique platform to study GA movement at high-resolution, in real time and in living, intact plants. By applying this platform to the Arabidopsis thaliana endogenous bioactive gibberellin GA4, we measure kinetic parameters of its flow, such as decay length and velocity, in vivo.

SUBMITTER: Wexler S 

PROVIDER: S-EPMC6354844 | biostudies-other | 2019 Feb

REPOSITORIES: biostudies-other

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Characterizing gibberellin flow <i>in planta</i> using photocaged gibberellins.

Wexler Shira S   Schayek Hilla H   Rajendar Kandhikonda K   Tal Iris I   Shani Eilon E   Meroz Yasmine Y   Dobrovetsky Roman R   Weinstain Roy R  

Chemical science 20181120 5


Gibberellins (GAs) are ubiquitous plant hormones that coordinate central developmental and adaptive growth processes in plants. Accurate movement of GAs throughout the plant from their sources to their destination sites is emerging to be a highly regulated and directed process. We report on the development of novel photocaged gibberellins that, in combination with a genetically encoded GA-response marker, provide a unique platform to study GA movement at high-resolution, in real time and in livi  ...[more]

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