Unknown

Dataset Information

0

Chaperone-mediated autophagy is involved in the execution of ferroptosis.


ABSTRACT: Necroptosis and ferroptosis are two distinct necrotic cell death modalities with no known common molecular mechanisms. Necroptosis is activated by ligands of death receptors such as tumor necrosis factor-α (TNF-α) under caspase-deficient conditions, whereas ferroptosis is mediated by the accumulation of lipid peroxides upon the depletion/or inhibition of glutathione peroxidase 4 (GPX4). The molecular mechanism that mediates the execution of ferroptosis remains unclear. In this study, we identified 2-amino-5-chloro-N,3-dimethylbenzamide (CDDO), a compound known to inhibit heat shock protein 90 (HSP90), as an inhibitor of necroptosis that could also inhibit ferroptosis. We found that HSP90 defined a common regulatory nodal between necroptosis and ferroptosis. We showed that inhibition of HSP90 by CDDO blocked necroptosis by inhibiting the activation of RIPK1 kinase. Furthermore, we showed that the activation of ferroptosis by erastin increased the levels of lysosome-associated membrane protein 2a to promote chaperone-mediated autophagy (CMA), which, in turn, promoted the degradation of GPX4. Importantly, inhibition of CMA stabilized GPX4 and reduced ferroptosis. Our results suggest that activation of CMA is involved in the execution of ferroptosis.

SUBMITTER: Wu Z 

PROVIDER: S-EPMC6386716 | biostudies-other | 2019 Feb

REPOSITORIES: biostudies-other

altmetric image

Publications

Chaperone-mediated autophagy is involved in the execution of ferroptosis.

Wu Zheming Z   Geng Yang Y   Lu Xiaojuan X   Shi Yuying Y   Wu Guowei G   Zhang Mengmeng M   Shan Bing B   Pan Heling H   Yuan Junying J  

Proceedings of the National Academy of Sciences of the United States of America 20190204 8


Necroptosis and ferroptosis are two distinct necrotic cell death modalities with no known common molecular mechanisms. Necroptosis is activated by ligands of death receptors such as tumor necrosis factor-α (TNF-α) under caspase-deficient conditions, whereas ferroptosis is mediated by the accumulation of lipid peroxides upon the depletion/or inhibition of glutathione peroxidase 4 (GPX4). The molecular mechanism that mediates the execution of ferroptosis remains unclear. In this study, we identifi  ...[more]

Similar Datasets

| S-EPMC7801434 | biostudies-literature
| S-EPMC5563711 | biostudies-other
| S-EPMC2945256 | biostudies-literature
| S-EPMC3609872 | biostudies-literature
| S-EPMC5584305 | biostudies-literature
| S-EPMC3879702 | biostudies-other
| S-EPMC4000261 | biostudies-literature
| S-EPMC4506737 | biostudies-literature
| S-EPMC3633160 | biostudies-literature
| S-EPMC524731 | biostudies-literature