Dataset Information

Capacitive Saccharide Sensor Based on Immobilized Phenylboronic Acid with Diol Specificity.

ABSTRACT: A capacitive sensor for saccharide detection is described in this study. The detection is based on selective interaction between diols and aminophenylboronic acid (APBA) immobilized on a gold electrode. Glucose, fructose, and dextran (MW: 40 kDa) were tested with the system over wide concentration ranges (1.0 x 10-8 M - 1.0 x 10-3 M for glucose, 1.0 x 10-8 M - 1.0 x 10-2 M for fructose and 1.0 x 10-10 M - 1.0 x 10-5 M for dextran). The limits of detection (LODs) were 0.8 nM for glucose, 0.6 nM for fructose, and 13 pM for dextran. These data were comparable to the others reported previously. In order to demonstrate glycoprotein detection with the same sensor, human immunoglobulin G (IgG) as well as horseradish peroxidase were used as model analytes. The sensor responded to IgG in the concentration range of 1.0 x 10-13 M - 1.0 x 10-7 M with a LOD value of 16 fM. The performance of the assay of peroxidase was compared to a spectrophotometric assay by determining the enzymatic activity of a captured analyte. The results showed that the method might be useful for label-free, fast, and sensitive detection of saccharides as well as glycoproteins over a wide concentration range.

SUBMITTER: Bergdahl GE

PROVIDER: S-EPMC6509085 | biostudies-other | 2019 May

REPOSITORIES: biostudies-other

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Capacitive Saccharide Sensor Based on Immobilized Phenylboronic Acid with Diol Specificity.

Bergdahl Gizem Ertürk GE Hedström Martin M Mattiasson Bo B

Applied biochemistry and biotechnology 20181028 1

A capacitive sensor for saccharide detection is described in this study. The detection is based on selective interaction between diols and aminophenylboronic acid (APBA) immobilized on a gold electrode. Glucose, fructose, and dextran (MW: 40 kDa) were tested with the system over wide concentration ranges (1.0 x 10-8 M - 1.0 x 10-3 M for glucose, 1.0 x 10-8 M - 1.0 x 10-2 M for fructose and 1.0 x 10-10 M - 1.0 x 10-5 M for dextran) ...[more]

PMID: 30370445

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Project description:Phenylboronic acids have emerged as synthetic receptors that can reversibly bind to cis-diols of glucose molecules. The incorporation of phenylboronic acids in hydrogels offers exclusive attributes; for example, the binding process with glucose induces Donnan osmotic pressure resulting in volumetric changes in the matrix. However, their practical applications are hindered because of complex readout approaches and their time-consuming fabrication processes. Here, we demonstrate a microimprinting method to fabricate densely packed concavities in phenylboronic acid functionalized hydrogel films. A microengineered optical diffuser structure was imprinted on a phenylboronic acid based cis-diol recognizing motif prepositioned in a hydrogel film. The diffuser structure engineered on the hydrogel was based on laser-inscribed arrays of imperfect microlenses that focused the incoming light at different focal lengths and direction resulting in a diffused profile of light in transmission and reflection readout modes. The signature of the dimensional modulation was detected in terms of changing focal lengths of the microlenses due to the volumetric expansion of the hydrogel that altered the diffusion spectra and transmitted beam profile. The transmitted optical light spread and intensity through the sensor was measured to determine variation in glucose concentrations at physiological conditions. The sensor was integrated in a contact lens and placed over an artificial eye. Artificial stimulation of variation in glucose concentration allowed quantitative measurements using a smartphone's photodiode. A smartphone app was utilized to convert the received light intensity to quantitative glucose concentration values. The developed sensing platform offers low cost, rapid fabrication, and easy detection scheme as compared to other optical sensing counterparts. The presented detection scheme may have applications in wearable real-time biomarker monitoring devices at point-of-care settings.

Dataset Information

Capacitive Saccharide Sensor Based on Immobilized Phenylboronic Acid with Diol Specificity.

Publications

Capacitive Saccharide Sensor Based on Immobilized Phenylboronic Acid with Diol Specificity.

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