Ontology highlight
ABSTRACT:
SUBMITTER: Dandage R
PROVIDER: S-EPMC6553823 | biostudies-other | 2019 Jun
REPOSITORIES: biostudies-other
Genetics 20190401 2
CRISPR-mediated base editors have opened unique avenues for scar-free genome-wide mutagenesis. Here, we describe a comprehensive computational workflow called <i>beditor</i> that can be broadly adapted for designing guide RNA libraries with a range of CRISPR-mediated base editors, Protospacer Adjacent Motif (PAM) recognition sequences, and genomes of many species. Additionally, to assist users in selecting the best sets of guide RNAs for their experiments, <i>a priori</i> estimates of editing ef ...[more]