Dataset Information

MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording.

ABSTRACT: Optical fiber-mediated optogenetic activation and neuronal Ca2+ recording in combination with fMRI provide a multi-modal fMRI platform. Here, we developed an MRI-guided robotic arm (MgRA) as a flexible positioning system with high precision to real-time assist optical fiber brain intervention for multi-modal animal fMRI. Besides the ex vivo precision evaluation, we present the highly reliable brain activity patterns in the projected basal forebrain regions upon MgRA-driven optogenetic stimulation in the lateral hypothalamus. Also, we show the step-wise optical fiber targeting thalamic nuclei and map the region-specific functional connectivity with whole-brain fMRI accompanied by simultaneous calcium recordings to specify its circuit-specificity. The MgRA also guides the real-time microinjection to specific deep brain nuclei, which is demonstrated by an Mn-enhanced MRI method. The MgRA represents a clear advantage over the standard stereotaxic-based fiber implantation and opens a broad avenue to investigate the circuit-specific functional brain mapping with the multi-modal fMRI platform.

SUBMITTER: Chen Y

PROVIDER: S-EPMC6557837 | biostudies-other | 2019 Jun

REPOSITORIES: biostudies-other

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MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca<sup>2+</sup> recording.

Chen Yi Y Pais-Roldan Patricia P Chen Xuming X Frosz Michael H MH Yu Xin X Yu Xin X

Nature communications 20190610 1

Optical fiber-mediated optogenetic activation and neuronal Ca<sup>2+</sup> recording in combination with fMRI provide a multi-modal fMRI platform. Here, we developed an MRI-guided robotic arm (MgRA) as a flexible positioning system with high precision to real-time assist optical fiber brain intervention for multi-modal animal fMRI. Besides the ex vivo precision evaluation, we present the highly reliable brain activity patterns in the projected basal forebrain regions upon MgRA-driven optogenetic ...[more]

PMID: 31182714

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Dataset Information

MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording.

Publications

MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca<sup>2+</sup> recording.

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