Comparing Raman and NanoSIMS for heavy water labeling of single cells
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ABSTRACT: This study analyzed 543 Escherichia coli K12 cells grown in M9 minimal medium in the absence or presence of heavy water (deuterium oxide) at single cell resolution using confocal Raman microspectroscopy. Cells were grown in unamended media (0%) or media amended with either 15%, 30%, or 50% heavy water and fixed with 2% paraformaldehyde. Measurements were made on mirrored stainless steel.
SUBMITTER:
PROVIDER: S-MBRS8 | biostudies-other |
REPOSITORIES: biostudies-other
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