CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments

ABSTRACT: Stabilization of microtubule plus end-directed kinesin CENP-E at the metaphase kinetochores is important for chromosome alignment, but its mechanism remains unclear. Here, we show that CKAP5, a conserved microtubule plus tip protein, regulates CENP-E at kinetochores in human cells. Depletion of CKAP5 impairs CENP-E localization at kinetochores at metaphase plate and results in increased kinetochore-microtubule stability and attachment errors. Erroneous attachments are also supported by computational modelling. Analysis of CKAP5 knockout cancer cells of multiple tissue origins shows that CKAP5 is preferentially essential in aneuploid, chromosomally-unstable cells, and the sensitivity to CKAP5 depletion is correlated to that of CENP-E depletion. CKAP5 depletion leads to reduction in CENP-E

SUBMITTER: R., Bhagya Lakshmi 

PROVIDER: S-SCDT-10_1038-S44319-024-00106-9 | biostudies-other |

REPOSITORIES: biostudies-other