The polyA tail facilitates splicing of last introns with weak 3´ splice sites via PABPN1

ABSTRACT: The polyA tail of mRNAs is important for many aspects of RNA metabolism. However, whether and how it regulates pre-mRNA splicing is still unknown. Here, we report that the polyA tail acts as a splicing enhancer for the last intron via the nuclear polyA binding protein PABPN1 in HeLa cells. PABPN1-depletion induces the retention of a group of introns with a weaker 3´ splice site, and they show a strong 3´-end bias and mainly locate in nuclear speckles. The polyA tail is essential for PABPN1-enhanced last intron splicing and functions in a length-dependent manner. Tethering PABPN1 to non-polyadenylated transcripts also promotes splicing, suggesting a direct role for PABPN1 in splicing regulation. Using TurboID-MS, we construct the PABPN1 interactome, including many spliceosomal and RNA-bindi

SUBMITTER: Li Huang 

PROVIDER: S-SCDT-10_15252-EMBR_202357128 | biostudies-other |

REPOSITORIES: biostudies-other