APEX2-mediated RAB proximity labeling identifies a role for RAB21 in clathrin-independent cargo sorting

ABSTRACT: RAB GTPases are central modulators of membrane trafficking. They are under the dynamic regulation of activating guanine exchange factors (GEFs) and inactivating GTPase activating proteins (GAPs). Once activated, RABs recruit a large spectrum of effectors to control trafficking functions of eukaryotic cells. Multiple proteomic studies, using pull-down or yeast two-hybrid approaches, have identified a number of RAB interactors. However, due to the in vitro nature of these and to inherent limitations of each technique, a comprehensive definition of RAB interactors is still lacking. By comparing quantitative affinity purifications of GFP:RAB21 with APEX2-mediated proximity labeling of RAB4a, RAB5a, RAB7a and RAB21, we find that APEX2 proximity labeling allows for the comprehensive identificati

SUBMITTER: Tomas Del Olmo 

PROVIDER: S-SCDT-91748_2_1551690886_jats | biostudies-other |

REPOSITORIES: biostudies-other