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4 RABs proxymity labelling - APEX2-mediated RAB proximity labeling identifies a role for RAB21 in clathrin-independent cargo sorting

ABSTRACT: APEX2-mediated proximity labeling was performed with 4 endosomal RABs allowed an efficient and comprehensive identification of their protein neighbors. APEX2-mediated proximity labeling was performed in triplicate. Protein enrichments obtained from all RABs were compared to APEX2-nude proximity labeling results. Finally, bioinformatic and biochemical approaches were used to confirm functional validity of RAB protein neighbor’s identification.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Tomas Del Olmo

LAB HEAD: steve jean

PROVIDER: PXD010950 | Pride | 2019-01-07

REPOSITORIES: Pride

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APEX2-mediated RAB proximity labeling identifies a role for RAB21 in clathrin-independent cargo sorting.

Del Olmo Tomas T Lauzier Annie A Normandin Caroline C Larcher Raphaëlle R Lecours Mia M Jean Dominique D Lessard Louis L Steinberg Florian F Boisvert François-Michel FM Jean Steve S

EMBO reports 20190103 2

RAB GTPases are central modulators of membrane trafficking. They are under the dynamic regulation of activating guanine exchange factors (GEFs) and inactivating GTPase-activating proteins (GAPs). Once activated, RABs recruit a large spectrum of effectors to control trafficking functions of eukaryotic cells. Multiple proteomic studies, using pull-down or yeast two-hybrid approaches, have identified a number of RAB interactors. However, due to the <i>in vitro</i> nature of these approaches and inh ...[more]

PMID: 30610016

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