Unknown

Dataset Information

0

A microtubule-LUZP1 association around tight junction promotes epithelial cell apical constriction


ABSTRACT: Apical constriction is critical for epithelial morphogenesis, including neural tube formation. Vertebrate apical constriction is induced by di-phosphorylated myosin light chain (ppMLC)-driven contraction of actomyosin-based circumferential rings (CRs), also known as perijunctional actomyosin rings (PAMRs), around apical junctional complexes (AJCs), mainly consisting of tight junctions (TJs) and adherens junctions (AJs). Here, we revealed a ppMLC-triggered system at TJ-associated CRs for vertebrate apical constriction involving microtubules, LUZP1, and myosin phosphatase. We first identified LUZP1 via unbiased screening of microtubule-associated proteins in the AJC-enriched fraction. In cultured epithelial cells, LUZP1 was found localized at TJ-, but not at AJ-, associated CRs, and LUZP1 knockout resulted in apical constriction defects with a significant reduction in ppMLC levels within CRs. A series of assays revealed that ppMLC promotes the recruitment of LUZP1 to TJ-associated CRs, where LUZP1 spatiotemporally inhibits myosin phosphatase in a microtubule-facilitated manner. Our results uncovered a hitherto unknown microtubule-LUZP1 association at TJ-associated CRs that inhibits myosin phosphatase, contributing significantly to the understanding of vertebrate apical constriction.

SUBMITTER: Dr. Tomoki Yano 

PROVIDER: S-SCDT-EMBOJ-2020-104712 | biostudies-other |

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC7809799 | biostudies-literature
| S-EPMC2895057 | biostudies-literature
| S-EPMC3392093 | biostudies-literature
| S-EPMC8101490 | biostudies-literature
| S-EPMC6829419 | biostudies-literature
| S-EPMC10281517 | biostudies-literature
| S-EPMC6504902 | biostudies-literature
| S-EPMC4022608 | biostudies-literature
| S-EPMC6179133 | biostudies-literature
2024-05-22 | PXD052221 | Pride