RNA localization and co-translational interactions control RAB13 GTPase function and cell migration

ABSTRACT: Numerous RNAs exhibit specific distribution patterns in mammalian cells. However, the functional and mechanistic consequences are relatively unknown. Here we investigate the functional role of RNA localization at cellular protrusions of migrating mesenchymal cells, using as a model the RAB13 RNA, which encodes a GTPase important for vesicle-mediated membrane trafficking. While RAB13 RNA is enriched at peripheral protrusions, the expressed protein is concentrated perinuclearly. By specifically preventing RAB13 RNA localization, we show that peripheral RAB13 translation is not important for the overall distribution of the RAB13 protein, or its ability to associate with membranes, but is required for full activation of the GTPase and for efficient cell migration. RAB13 translation leads to a

SUBMITTER: Dr. Konstadinos Moissoglu 

PROVIDER: S-SCDT-EMBOJ-2020-104958 | biostudies-other |

REPOSITORIES: biostudies-other