Mitotic checkpoint gene expression is tuned by codon usage bias

ABSTRACT: The mitotic checkpoint (also called spindle assembly checkpoint, SAC) is a signaling pathway that safeguards proper chromosome segregation. Correct functioning of the SAC depends on adequate protein concentrations and appropriate stoichiometries between SAC proteins. Yet very little is known about the regulation of SAC gene expression. Here, we show in the fission yeast S. pombe that a combination of short mRNA half-lives and long protein half-lives supports stable SAC protein levels. For the SAC genes mad2+ and mad3+, their short mRNA half-lives are caused, in part, by a high frequency of non-optimal codons. In contrast, mad1+ mRNA has a short half-life despite a higher frequency of optimal codons, and despite the lack of known RNA-destabilizing motifs. Hence, different SAC genes employ d

SUBMITTER: Mr. Eric Esposito 

PROVIDER: S-SCDT-EMBOJ-2021-107896 | biostudies-other |

REPOSITORIES: biostudies-other