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C-Maf enforces cytokine production and promotes memory-like responses in mouse and human type 2 Innate Lymphoid Cells

ABSTRACT: Group-2 innate lymphoid cells (ILC2s), which are involved in type-2 inflammatory diseases such as allergy, can exhibit immunological memory, but the basis of this ILC2 "trained immunity" has remained unclear. Here we found that stimulation with IL-33/IL-25 or exposure to the allergen papain induces the expression of the transcription factor c-Maf in mouse ILC2s. Chronic papain exposure results in high production of IL-5 and IL-13 cytokines and lung eosinophil recruitment, effects that are blocked by c-Maf deletion in ILCs. Transcriptomic analysis revealed that knock-down of c-Maf in ILC2s suppresses expression of type-2 cytokine genes, as well as of genes linked to a memory-like phenotype. Consistently, c-Maf was found highly expressed in human adult ILC2s but absent in cord blood, and required for cytokine production in isolated human ILC2s. Furthermore, c-Maf-deficient mouse or human ILC2s failed to exhibit strengthened ("trained") responses upon repeated challenge. Thus, the expression of c-Maf is indispensable for optimal type-2 cytokine production and proper memory-like responses in group-2 innate lymphoid cells.

SUBMITTER: Dr. Sara Trabanelli

PROVIDER: S-SCDT-EMBOJ-2021-109300 | biostudies-other |

REPOSITORIES: biostudies-other

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c-Maf enforces cytokine production and promotes memory-like responses in mouse and human type 2 innate lymphoid cells.

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