Single-cell transcriptomics identifies Gadd45b as a regulator of herpesvirus-reactivating neurons

ABSTRACT: Single-cell RNA sequencing (scRNA-seq) is a powerful technique for dissecting the complexity of normal and diseased tissues, enabling characterization of cell diversity and heterogeneous phenotypic states in unprecedented detail. However, this technology has been underutilized for exploring the interactions between the host cell and viral pathogens in latently-infected cells. Herein, we use scRNA-seq and single-molecule sensitivity fluorescent in situ hybridization (smFISH) technologies to investigate host single-cell transcriptome changes upon the reactivation of a human neurotropic virus, herpes simplex virus-1 (HSV-1). We identify the stress sensor Growth arrest and DNA damage-inducible 45 beta (Gadd45b) as a critical antiviral host factor that regulates HSV-1 reactivation events in a s

SUBMITTER: Hui-Lan Hu 

PROVIDER: S-SCDT-EMBOR-2021-53543-T | biostudies-other |

REPOSITORIES: biostudies-other