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Genetic Measurement of Memory B-cell Recall Using Antibody Repertoire Sequencing

ABSTRACT:

We developed an improved high throughput sequencing approach to measure the quantities and sequences of the repertoire of antibody heavy chain RNA in a blood sample. Using this approach we analyzed the antibody repertoire in response to yearly vaccinations with influenza vaccines TIV and LAIV in healthy adults in two subsequent years. We determined vaccine response patterns specific to LAIV and TIV and found antibody sequences that were shared between two samples of the same individuals following influenza vaccination in subsequent years, thereby providing a genetic measurement of B-cell memory recall.

PROVIDER: phs000656 | dbGaP |

SECONDARY ACCESSION(S): PRJNA216951PRJNA216953

REPOSITORIES: dbGaP

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Dataset's files

Source:

			Action	DRS
	GapExchange_phs000656.v1.p1.xml	Xml
	dbGaPEx2.1.5.xsd	Other
	Study_Report.phs000656.InfluenzaVaccination_Plasmablast_Antibody.v1.p1.MULTI.pdf	Pdf
	manifest_phs000656.InfluenzaVaccination_Plasmablast_Antibody.v1.p1.c1.IRU.pdf	Pdf
	datadict_v2.xsl	Other

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