Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drain Fluid Amylase as an early biomarker of anastomotic leak in low colorectal anastomoses

ABSTRACT: Interventions: Measure amylase levels in drain fluid daily after low colorectal anastomoses as an early indicator of anastomotic leak. - a small sample of drain fluid will be collected daily while pelvic drain is in situ during hospital admission following colorectal surgery and samples will be sent to the local pathology service for measurement of amylase - the amylase levels in drain fluid at each collection while the drain is in-situ will be collected and correlated to the clinical course - clinical course following surgery will be observed during admission (and data collected after patient has been discharged, retrospective data collection). - Follow up consultation at 6 weeks following surgery Primary outcome(s): Daily amylase levels in the drain fluid post-operatively. The measurement of amylase in drain fluid is measured and reported by local pathology service.[Day 1, Day 2, Day 3, Day 4 and Day 5, post operatively, or until pelvic drain is removed. ] Study Design: Purpose: Screening;Duration: Longitudinal;Selection: Defined population;Timing: Both

DISEASE(S): Surgery-other Surgery,Colorectal Cancer,Cancer-bowel-back Passage (rectum) Or Large Bowel (colon),Anastomotic Leak

PROVIDER: 2471651 | ecrin-mdr-crc |

REPOSITORIES: ECRIN MDR

ACCESS DATA

Json Xml

Similar Datasets

A Phase 2 study of efficacy: The validation of Gastrografin measurement in postsurgical samples following low anterior resection when the rectal tube is flushed with Gastrografin

Project description:Interventions: Patients who undergo low anterior resection without a diverting loop ileostomy and have a rectal tube and pelvic drain will be invited to participate. Only participants who are included in the study (by providing written consent) will receive Gastrografin flushes of rectal tube four times per day (QID), to replace saline flushes QID. At Day 1, Day 2, Day 3, Day 4, Day 5 (or until rectal tubes is in situ) after surgery: Participants will receive rectal tube flushing with 30 ml Gastrografin (25% v/v in saline) 4 times per day (QID). At 06:30am and 16:30pm, a 20 ml sample of drain fluid will be collected and stored until all samples during hospital admission are collected (for measurement of Gastrografin by DECT and ICPMS). Also, a sample from the rectal tube will be collected on Day 1 after surgery to confirm the presence of luminal Gastrografin. Patients will be observed during the hopital admission following surgery. The presence of Gastrografin will be measured in each sample by DECT and ICPMS. The clinical course post surgery will be monitored in all participants and the group will be catagorised into patients that DO NOT experience an anastomotic leak following surgery and patients that DO experience an anastomotic leak following surgery. Primary outcome(s): Level of Gastrografin in drain fluid will be measured by DECT and ICP-MS[Day 1, 2, 3, 4, 5 or until rectal tube is in situ];Anastomotic leak after surgery by clinical assessment and CT (as per standard care)[Day 1, 2, 3, 4, 5 post surgery] Study Design: Purpose: Diagnosis; Allocation: Non-randomised trial

| 2470927 | ecrin-mdr-crc

Prevention of post-operative pelvic bleeding and collection after sphincter-saving surgery for rectal cancer using a calcium-alginate haemostatic

Project description:Study hypothesis: Assess the possible benefit of Hemoionic, a new haemostatic, in sphincter-saving surgery for rectal cancer with special reference to pelvic fluid collection reduction. Primary outcome(s): Volume of fluid collected by the pelvic suction drain.

| 2423965 | ecrin-mdr-crc

Characterizing the wound healing response that drives human epidermal maturation on transcriptome level

Project description:We collected biopsies from patients undergoing split-thickness skin graft harvesting. The first biopsy was collected from the intact skin before wounding and the second and third from the donor site wound on 14th and 21st day post-operatively. A genome-wide microarray was then executed from each sample, and the gene profiles of each time point were compared.

2013-08-30 | GSE50425 | GEO

Copepod Diel Vertical Migration Proteomics

Project description:This project characterizes the metabolic consequences of the daily physiological rhythms and diel vertical migration for the model subtropical copepod, Pleuromamma xiphias. P. xiphias were collected near the Bermuda Atlantic Time Series in plankton tows at different times of day, representing different parts of their daily vertical migration. Single copepods were isolated from the tows and flash-frozen for proteomics analysis.

2024-01-14 | PXD021156 | Pride

The Pelvic Radiation and Vorinostat (PRAVO) Study - Histone Deacetylase Inhibitor Activity

Project description:The study objective was to propose molecular mechanisms of action of the histone deacetylase inhibitor vorinostat. In the PRAVO phase 1 study, patients that were scheduled to receive pelvic palliative radiation to 30 Gy in 3-Gy fractions for gastrointestinal carcinoma, were enrolled onto four sequential dose levels of vorinostat, starting at 100 mg daily with dose escalation in increments of 100 mg. Endpoints included treatment safety and tolerability, tumor response, and biological activity of vorinostat. For the purpose of identifying biomarkers of vorinostat action, peripheral blood mononuclear cells, representing normal tissue exposed to vorinostat, were used. The samples were collected, one at baseline and two on-treatment samples. The time points for sample collection were chosen based on our previous data from experimental colorectal carcinoma models exposed to vorinostat, demonstrating that the maximum tumor histone acetylation 2-3 hours after drug exposure was restored to baseline after 24 hours. In PRAVO study patients, tumor histone hyperacetylation was observed 3 hours after vorinostat administration. From the 17 patients enrolled onto the PRAVO study, a full set of three samples was obtained from 14 individuals: one baseline sample collected prior to commencement of vorinostat treatment (T0), and two on-treatment samples collected 2 and 24 hours after the patient had received the preceding daily dose of vorinostat (T2 and T24). Individual vorinostat dose levels were 100 mg (D100), 200 mg (D200), 300 mg (D300), or 400 mg (D400).

2013-05-08 | E-GEOD-46703 | biostudies-arrayexpress

Characterizing the wound healing response that drives human epidermal maturation on transcriptome level

2013-08-30 | E-GEOD-50425 | biostudies-arrayexpress

Eucalyptus xylem diurnal time series

Project description:The daily cycle of night and day affects the behaviour and physiology of almost all living things. At the molecular level, many genes show daily changes in expression levels. To determine whether changes in transcript abundance occur in wood forming tissues of Eucalyptus trees we used a cDNA microarray to examine gene expression levels at roughly four hour intervals throughout the day.

2009-05-13 | GSE11731 | GEO

Targeted proteomics of diel cycle of copepod Pleuromamma xiphias

Project description:The diel vertically migrating copepod, Pleuromamma xiphias, migrates approximately 600 m daily, up and down in the water column. At the surface, the copepods feed on plankton at night, and then descend to the depths during the day, likely to escape visual predators. Both the migratory behavior and the physiological pathways that are up- and down-regulated throughout the migration are likely under at least partial circadian clock control. This experiment compares the protein abundances of select physiological processes from copepods collected in situ with copepods incubated in the dark to remove the influence of exogenous stimuli on physiology.

2024-05-02 | PXD033033 | panorama

An evaluation of the Clear Fluids Diet: A comparison between patients commencing on Clear Fluids or Free Fluids and the effect this has on post-operative outcomes after elective large bowel surgery.

Project description:Interventions: Patients commence on sips of water ad libitum day 1 post-operatively. Once the treating surgeon deems the patient appropriate to commence fluids, they will start on Free Fluids ad libitum, (usually for 1-4 days), then Light Diet ad libitum (1-2 days) and then to Full Diet ad libitum (until discharged) Free Fluids are defined as low fibre foods which are fluid like in nature at room temperature including, all Clear Fluids, milk and soy based drinks and nutrition supplements, strained porridge, pureed soup, ice-cream, plain yoghurt and custard Primary outcome(s): Number of days of hospital admission post surgery collected from medical records.[At discharge from Frankston Hospital];Post-operative complications including ileus, anastomotic breakdown and wound breakdown as documented in the medical history by the medical team. Pneumonia (from chest xray showing positive infiltrates and positive sputum culture), urinary tract infection (positive urine cultures), sepsis (temperature greater than 38 degrees, heart rate above 90 bpm, respiratory rate greater that 20 rpm and white cell count above 12 x10^9/L or less than 4 x10^9/L or more than 10 % band forms in the absence of chemotherapy induced neutropenia or leukopenia) and mortality from medical history.[Incidence of complications will be collected during hospital admission];Proportion of nutritional requirements met using food and fluid chart analysis. Nutritional requirements will be calculated using the Schofield Equation.[Until patient is tolerating Light Diet for 24 hours] Study Design: Purpose: Treatment; Allocation: Randomised controlled trial

| 2455275 | ecrin-mdr-crc

Early predictive factors of mortality in onco-hematology patients admitted to the Intensive Care Unit for septic shock: A proteomic approach.

Project description:The number of organ failures at intensive care unit (ICU) admission is the main prognostic factor in septic shock. The aim was to assess classical clinico-biological parameters evaluating organ dysfunctions at ICU admission, combined with proteomics analysis, on day-30 mortality in critically ill onco-hematology patients admitted to the ICU for septic shock.

2018-08-22 | PXD004001 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data