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Study on change of PBMC and tumor local immune map in colon cancer patients before and after general anesthesia based on single cell transcriptome sequencing

ABSTRACT: Interventions: Sevoflurane group:Nil;Propofol group:NIl Primary outcome(s): Heterogeneity of immune cell RNA spectrum in local tissue and peripheral blood of tumor Study Design: Non randomized control

DISEASE(S): Colorectal Cancer

PROVIDER: 2706736 | ecrin-mdr-crc |

REPOSITORIES: ECRIN MDR

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RNA sequencing of young mice hippocampus after anesthesia

Project description:We performed RNA sequencing for hippocampus of neonatal mice that were exposed to sevoflurane. We treated a group of neonatal mice with 2.5% sevoflurane for 2 hours on day 6, 7, 8, 9 and treated another group on day 6, 7. On day 36, we collected RNA (both total RNA and polysome-associated RNA) from the hippocampus in the aforementioned two groups and the control group (i.e., without sevoflurane treatment). We prepared the sequencing libraries with the collected RNA and sent them to Illumina sequencing platform.

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A single-cell RNA-seq survey of the action profile of general anaesthetics on the human prefrontal cortex cells

Project description:The cellular and molecular actions of general anaesthetics to induce anaesthesia state and also cellular signalling changes for subsequent potential “long term” effects remain largely elusive although great efforts have been made to study these in vitro, ex vivo and in vivo settings. General anaesthetics were reported to act on voltage-gated ion channels and ligand-gated ion channels. Here we used single-cell RNA-sequencing complemented with whole-cell patch clamp and calcium transient techniques to examine the gene transcriptome and ion channels profiling of sevoflurane and propofol, both commonly used clinically, on human embryonic primary prefrontal cortex (PFC) mixed cell cultures. Both propofol and sevoflurane at clinically relevant dose/concentration promoted “microgliosis” but only sevoflurane changed microglia cell similarity. Propofol and sevoflurane each extensively but transiently altered transcriptome profiling 2 hours after anaesthetics exposure across microglia, excitatory neurons, interneurons, astrocytes and oligodendrocyte progenitor cells. Within the excitatory neurons and microglia, exemplary ion-gated and ligand-gated ion channels related genes response to either anaesthetic included SCN1A, CACNAB2, KCNA1, GABRR2 and GRINA1 amongst many others. Utilising scRNA-seq as a robust and high-throughput tool, our work may provide a comprehensive blueprint for future mechanistic studies of general anaesthetics in clinically relevant settings.

2023-05-03 | GSE196239 | GEO

Expression data from glioblastoma surgical specimens

Project description:Surgical resection is the first choice of the standardized treatment scheme for glioblastoma and is performed under general anesthesia. Previous researches have shown that propofol and sevoflurane have different effects on the biological process of glioma cells from the molecular level. Some of them, for instance, control the glioma cell proliferation, invasion and migration In vitro or In vivo. We used mRNA microarray to discuss the effects of propofol and sevoflurane on gene expression in patients with glioblastoma. The correlation between DEGs and patients’ prognosis was further analyzed.

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Comparison of global gene expression profile associated with inhalation anesthesia between sevoflurane and desflurane

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Effects of sevoflurane inhalation anesthesia on proliferation and apoptosis of colon cancer cells in vitro: a randomized controlled trial.

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Cancer and Anesthesia: Survival After Radical Surgery - a Comparison Between Propofol or Sevoflurane Anesthesia

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Protective effect of remote ischemic preconditioning combined with sevoflurane posttreatment on myocardial injury during laparoscopic radical resection of colorectal cancer

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Astrocytes modulate a specific paraventricular thalamus-prefrontal cortex projection to enhance consciousness recovery from sevoflurane anesthesia in mice

Project description:Previous studies have proved that astrocytes may be a key neural substrate that regulates wakefulness and consciousness recovery from general anesthesia, while the exact molecular target in astrocytes is still unclear. Using virus injection and in vivo fiber photometry in mice, we found both activating astrocytes and knocking down astrocytic Kir4.1 in paraventricular thalamus (PVT) promotes the consciousness recovery from sevoflurane anesthesia. Single-cell RNA sequencing of PVT reveals two distinct cellular subtypes of glutamatergic neurons: PVTGRM and PVTChAT neurons. Patch-clamp recording results proved that Astrocytic Kir4.1-mediated modulation of sevoflurane on PVT mainly works on PVTChAT neurons. Moreover, we found that PVTChAT neurons project mainly to the mPFC. This specific paraventricular thalamus to prefrontal cortex projection is involved in recovery of consciousness from sevoflurane anesthesia indirectly through modulation of astrocytes. In summary, our findings support the novel conception that the volatile anesthetic sevoflurane can inhibit PVT astrocytic Kir 4.1 to maintain and/or increase neuronal firing of PVTChAT neurons, which mainly projects to mPFC and promotes consciousness recovery from anesthesia.

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Expression data from sevoflurane anesthesia treated rat

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