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40065379-a291-4b20-8078-a332e01f7dcb - samples

ABSTRACT: SPECTA Lung cancer RNA FASTQ files (Illumina TST170 targeted analysis)

PROVIDER: EGAD00001006222 | EGA |

REPOSITORIES: EGA

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d880baae-2ceb-4cc2-9de0-05b00febf89d - samples

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Project description:Purpose: To identify differential mRNA expression in response to SHH stimulation in murine embryonic and lung fibroblasts Methods: NIH-3T3 and MLg cell lines were treated with recombinant SHH or vehicle control and RNA-seq was performed using Illumina Miseq Nano (performed by Admera Health, LLC (South Plainfield, NJ)). The FASTQ files were checked for quality using FastQC (v0.11.2). FASTQ files were mapped to the mm10 assembly of the mouse genome using TopHat; fragments per kilo base per million mapped reads (FPKM) calculation and differential expression analysis were performed using Cufflinks (v 2.2.1). Results: Comparison of differentially up-regulated and down-regulated mRNAs of secretory protein genes in NIH-3T3 and MLg cell lines upon SHH activation revealed interesting differences in the production of some ligands.

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High-throughput RNA Sequencing of THP-1 macrophages treated with or without native LDL for 24h.

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LCLF1.0 fastq-Files

Project description:LCLF1.0 fastq-Files

| PRJNA928833 | ENA

LCLF1.0 fastq-Files

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a1a95e0d-c7f6-4e0b-8d13-345a7c9d7f79 - samples

Project description:SPECTA Lung cancer VCF files

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Differential expression data between E15.5 outflow tracts of Gata4 G295S mutant embryos and littermate controls

Project description:E15.5 embryos were micro-disscted from Gata4 G295S mutant mice and littermate controls, RNA was isolated using Norgen total RNA isolation, and libraries were generated with the RNA TruSeq Stranded Total RNA kit. 50 base pair paired end reads were obtained on an illumina high seq 2500. Fastq files were aligned to the mouse genome using STAR aligner. QC was performed using RNASeQC and RSeQC. BAM files were processed using cufflinks pipeline.

2019-05-17 | GSE117621 | GEO

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