Project description:Purpose: To identify differential mRNA expression in response to SHH stimulation in murine embryonic and lung fibroblasts Methods: NIH-3T3 and MLg cell lines were treated with recombinant SHH or vehicle control and RNA-seq was performed using Illumina Miseq Nano (performed by Admera Health, LLC (South Plainfield, NJ)). The FASTQ files were checked for quality using FastQC (v0.11.2). FASTQ files were mapped to the mm10 assembly of the mouse genome using TopHat; fragments per kilo base per million mapped reads (FPKM) calculation and differential expression analysis were performed using Cufflinks (v 2.2.1). Results: Comparison of differentially up-regulated and down-regulated mRNAs of secretory protein genes in NIH-3T3 and MLg cell lines upon SHH activation revealed interesting differences in the production of some ligands.

2022-02-13 | GSE126596 | GEO

2083b4f7-ce4e-453c-ad96-bd5344e43ae1 - samples

Project description:TST170 DNA FASTQ files

| EGAD00001005502 | EGA

Tonsil Atlas FASTQ files

Project description:Tonsil Atlas FASTQ files

| PRJEB75429 | ENA

a1a95e0d-c7f6-4e0b-8d13-345a7c9d7f79 - samples

Project description:SPECTA Lung cancer VCF files

| EGAD00001006260 | EGA