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52f16dbc-e837-4156-903b-8fb108ee33e8 - samples

ABSTRACT: Whole Exome Sequencing of blood and FFPE tumour of patient #368. Ã¢Â€Â¯150Ã¢Â€Â“300 ng of DNA was fragmented to approximately 200 bp using a focal acoustic device. Libraries were prepared with the Kapa Hyper Prep Kit and SureSelectXT adaptors. Hybridisation capture was performed with SureSelect Clinical Research Exome V2 baits following the SureSelectXT recommended protocol (Agilent). Indexed libraries were sequenced on an Illumina NovaSeq 6000 to generate paired-end 150 bp reads with average of 70-fold base coverage for the germline sample and 330-fold coverage for tumour sample.

PROVIDER: EGAD00001011272 | EGA |

REPOSITORIES: EGA

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