WTCCC3_Pre-Eclampsia
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ABSTRACT:
PROVIDER: EGAD00010000854 | EGA |
REPOSITORIES: EGA
Publications
Genetic predisposition to hypertension is associated with preeclampsia in European and Central Asian women.
Steinthorsdottir Valgerdur V McGinnis Ralph R Williams Nicholas O NO Stefansdottir Lilja L Thorleifsson Gudmar G Shooter Scott S Fadista João J Sigurdsson Jon K JK Auro Kirsi M KM Berezina Galina G Borges Maria-Carolina MC Bumpstead Suzannah S Bybjerg-Grauholm Jonas J Colgiu Irina I Dolby Vivien A VA Dudbridge Frank F Engel Stephanie M SM Franklin Christopher S CS Frigge Michael L ML Frisbaek Yr Y Geirsson Reynir T RT Geller Frank F Gretarsdottir Solveig S Gudbjartsson Daniel F DF Harmon Quaker Q Hougaard David Michael DM Hegay Tatyana T Helgadottir Anna A Hjartardottir Sigrun S Jääskeläinen Tiina T Johannsdottir Hrefna H Jonsdottir Ingileif I Juliusdottir Thorhildur T Kalsheker Noor N Kasimov Abdumadjit A Kemp John P JP Kivinen Katja K Klungsøyr Kari K Lee Wai K WK Melbye Mads M Miedzybrodska Zosia Z Moffett Ashley A Najmutdinova Dilbar D Nishanova Firuza F Olafsdottir Thorunn T Perola Markus M Pipkin Fiona Broughton FB Poston Lucilla L Prescott Gordon G Saevarsdottir Saedis S Salimbayeva Damilya D Scaife Paula Juliet PJ Skotte Line L Staines-Urias Eleonora E Stefansson Olafur A OA Sørensen Karina Meden KM Thomsen Liv Cecilie Vestrheim LCV Tragante Vinicius V Trogstad Lill L Simpson Nigel A B NAB Aripova Tamara T Casas Juan P JP Dominiczak Anna F AF Walker James J JJ Thorsteinsdottir Unnur U Iversen Ann-Charlotte AC Feenstra Bjarke B Lawlor Deborah A DA Boyd Heather Allison HA Magnus Per P Laivuori Hannele H Zakhidova Nodira N Svyatova Gulnara G Stefansson Kari K Morgan Linda L
Nature communications 20201125 1
Preeclampsia is a serious complication of pregnancy, affecting both maternal and fetal health. In genome-wide association meta-analysis of European and Central Asian mothers, we identify sequence variants that associate with preeclampsia in the maternal genome at ZNF831/20q13 and FTO/16q12. These are previously established variants for blood pressure (BP) and the FTO variant has also been associated with body mass index (BMI). Further analysis of BP variants establishes that variants at MECOM/3q ...[more]
Similar Datasets
Project description:Genome-wide analysis of decidual transcriptome in pre-eclampsia compared with normotensive controls to find differentially expressed genes/pathways. Preeclampsia (PE) is a common and serious pregnancy hypertensive disorder with a strong genetic component. The study aims were to use genome-wide analysis of decidual transcriptome in pre-eclampsia compared with normotensive controls to identify differentially expressed biological pathways, and to determine common pathways between the transcriptome and previously identified maternal susceptibility genes.
2015-08-05 | GSE60438 | GEO
Project description:Genome-wide analysis of decidual transcriptome in pre-eclampsia compared with normotensive controls to find differentially expressed genes/pathways. Preeclampsia (PE) is a common and serious pregnancy hypertensive disorder with a strong genetic component. The study aims were to use genome-wide analysis of decidual transcriptome in pre-eclampsia compared with normotensive controls to identify differentially expressed biological pathways, and to determine common pathways between the transcriptome and previously identified maternal susceptibility genes. Total RNA from decidua basalis obtained from pre-eclamptic and normotensive control patients at Caesarean section
2015-08-05 | E-GEOD-60438 | biostudies-arrayexpress
Project description:To determine differential expression of microRNAs in placentae with severe pre-eclampsia and normal placenta. Differential expression of microRNAs in placentae (4 severe pre-eclampsia and 4 normal control) was screened by microarray platform, then some differential microRNAs were selected and validated with real-time quantitative reverse transcription polymerase chain reaction in placentae of severe pre-eclampsia (n=24) and normal control group (n=26). Results: We validated the expression of some microRNAs altered in the microarray, and found the following microRNAs were significantly increased in severe pre-eclampsia placentae: miR-16, miR-29b, miR-195, miR-26b, miR-181a, miR-335 and miR-222. Conclusion: These differential microRNAs may play an important role in pathogenesis of pre-eclampsia.
2011-04-14 | GSE15789 | GEO
Project description:Pre-eclampsia is one of the most serious pregnancy-associated disorders, and is defined by hypertension (systolic blood pressure higher than 140mmHg or diastolic blood pressure higher than 90mmHg) with proteinuria (more than 0.3g/day). It is not a simple complication of pregnancy, but is rather a syndrome of multiple organ failure involving the liver, kidney, and lung, as well as coagulatory and neural systems. There is now an emerging consensus that pre-eclampsia is a complex polygenetic trait in which maternal and fetal genes, as well as environmental factors, are involved, although the precise mechanism of the disorder has remained elusive. In this study, we have performed gene expression profiling to further elucidate the mechanisms underlying the development of the pre-eclamptic condition. We thus compared the expression profiles in placentas from women who underwent a normal pregnancy and from women suffering from severe pre-eclampsia. In addition, we compared the expression data between the early and late onset forms of pre-eclampsia and obtained a number of potential new prognostic biomarkers for this disease. Keywords: disease state analysis
2006-04-25 | GSE4707 | GEO
Project description:Maternal Blood histamine levels are tightly controlled in normal pregnancy. However, in specific complications of human pregnancy such as pre-eclampsia the levels of both placental and maternal blood histamine increase. Increasing blood histamine levels nonetheless, have been associated with oxidative stress, endothelial dysfunction, abnormal tissue growth, and Th1/TH2 imbalance, which are also linked to pre-eclampsia. Little is known of the molecular responses in the placenta to the prolonged exposure to increasing histamine levels in the presence of changing oxygen concentrations. We used microarray to detail the global programme of placental gene expression in response to histamine and oxygen and identified distinct classes of regulated genes underlying the molecular functions of histamine in the placenta.
2017-06-01 | GSE74446 | GEO
Project description:To determine differential expression of microRNAs in placentae with severe pre-eclampsia and normal placenta. Differential expression of microRNAs in placentae (4 severe pre-eclampsia and 4 normal control) was screened by microarray platform, then some differential microRNAs were selected and validated with real-time quantitative reverse transcription polymerase chain reaction in placentae of severe pre-eclampsia (n=24) and normal control group (n=26). Results: We validated the expression of some microRNAs altered in the microarray, and found the following microRNAs were significantly increased in severe pre-eclampsia placentae: miR-16, miR-29b, miR-195, miR-26b, miR-181a, miR-335 and miR-222. Conclusion: These differential microRNAs may play an important role in pathogenesis of pre-eclampsia. We analyzed the microRNA expression in the placentae of Chinese patients with severe pre-eclampsia.
2011-04-14 | E-GEOD-15789 | biostudies-arrayexpress
Project description:Pre-eclampsia is one of the most serious pregnancy-associated disorders, and is defined by hypertension (systolic blood pressure higher than 140mmHg or diastolic blood pressure higher than 90mmHg) with proteinuria (more than 0.3g/day). It is not a simple complication of pregnancy, but is rather a syndrome of multiple organ failure involving the liver, kidney, and lung, as well as coagulatory and neural systems. There is now an emerging consensus that pre-eclampsia is a complex polygenetic trait in which maternal and fetal genes, as well as environmental factors, are involved, although the precise mechanism of the disorder has remained elusive. In this study, we have performed gene expression profiling to further elucidate the mechanisms underlying the development of the pre-eclamptic condition. We thus compared the expression profiles in placentas from women who underwent a normal pregnancy and from women suffering from severe pre-eclampsia. In addition, we compared the expression data between the early and late onset forms of pre-eclampsia and obtained a number of potential new prognostic biomarkers for this disease. Experiment Overall Design: Placental biopsies were obtained during Caesarean section from both normotensive patients and from those with pre-eclampsia (n = 14) (early onset type; earlier than 31 weeks gestation, n = 6 and late onset type; 31 weeks gestation or later, n = 8). Pre-eclampsia was defined as a blood pressure of higher than 160/110 mmHg, with proteinuria of more than 2g in a 24 hour collection. The expression profiles of approximately 47,669 genes were analyzed using a Whole Human Genome Oligo Microarray Kit (Agilent Technologies). A total of 10 placentas from women with pre-eclampsia and four from normal subjects were used in the hybridizations. Reverse transcription labeling and hybridization was performed using the protocol recommended by the manufacturer. The microarray experiments were then carried out using competitive hybridization experiments with Cy3 and Cy5-labeled targets; one with test placental RNAs from patients or normal control subjects, and another using pooled normal placental RNA as a template control for normalization. The glass slides were scanned using an Agilent G2565BA microarray scanner. Scanned images were then analyzed using Feature Extraction software. The average signal intensities were corrected for median background intensity and transferred with GenBank descriptors to a Microsoft Excel data spreadsheet (Microsoft, Redmond, WA). Data analysis was performed using Genespring software version 6.1 (Silicon Genetics, Redwood City, CA). After intensity dependent normalization (Lowess), the expression levels relative to the control were calculated as a ratio, and the expression profiles were then compared between each pre-eclamptic or normal sample.
2008-05-07 | E-GEOD-4707 | biostudies-arrayexpress
Project description:An allorecognition system depending on interactions between uterine Natural Killer (uNK) cells and placental extravillous trophoblast (EVT) during early pregnancy influences reproductive outcomes in humans. Our previous immunogenetic studies show that particular combinations of maternal Killer Immunoglobulin-like Receptors (KIR) in uNK cells with fetal HLA-C variants on EVT are associated with disorders of pregnancy, especially pre-eclampsia. To identify responses stimulated in uNK cells specifically when activating KIR (KIR2DS1) binds to C2+HLA-C, a combination protective against pre-eclampsia, we modelled KIR-HLA interactions by co-culturing uNK cells with the 721.221 HLA-null cell line transfected to express either C1+ or C2+HLA-C molecules, followed by transcriptome profiling by RNA sequencing. We detect the secretion of key cytokines by uNK cells under the protective combination between KIR2DS1 and C2+HLA-C.
2024-01-10 | E-MTAB-13379 | biostudies-arrayexpress
Project description:The high incidence of pre-eclampsia, affecting 5-10% of pregnancies, makes it a major health problem. Early detection of pre-eclampsia, before the appearance of the first clinical symptoms, is therefore an urgent need, since it would allow the early intervention. Identification of plasma/serum biomarkers would pave the way to develop new strategies for the diagnosis of pre-eclampsia. Liquid biopsy emerges as a promising source of protein biomarkers that circumvent some of the inherent challenges of proteome wide analysis of plasma and serum. Besides, purified exosomes have the added interest of being communication vehicles between cells and tissues both in physiological and pathological processes. Methods We compared the protein abundance in purified exosomes from three different cohorts of control and preeclamptic serum samples, obtained around the sixth month and at the end of pregnancy. To this end, a shotgun label-free proteomics analysis was conducted and the relevant differential proteins were then validated by targeted MRM. Results An exosome purification method was developed that yielded highly enriched preparations, as indicated by the detection of a large number of exosomal markers. Likewise, the presence of specific pregnancy protein markers suggested that a very significant percentage of purified exosomes come from tissues related to pregnancy. Shotgun quantitative proteomic analysis allowed us to identify 10, 114 and 98 differentially regulated proteins in the three studied cohorts, with a high degree of concordance among them. Functional analysis suggests that these proteins participate in biological processes closely related to pre-eclampsia, such as angiogenesis, inflammation or cell migration. Differential abundance of 66 proteins were validated by MRM. Finally, we show the impact of the pre-eclampsia associated exosomes in the proteome of a cellular model, compared to normal exosomes. Conclusions We identified and validated differential proteins in liquid biopsy of patients that open new possibilities for the early diagnostic of pre-eclampsia. Additionally, the functional impact of the different proteome composition of purified pre-eclamptic exosomes in target cells provide new information to further understand changes on embryo-maternal interactions and therefore the pathogenesis of this disease.
2022-02-21 | PXD025308 | Pride