Project description:1. The expression of plasma exosomal ncRNAs and mRNAs was compared a group of 12 samples from healthy individuals (normal group) with a group of 30 samples from cervical cancer patients before primary concurrent chemoradiotherpay (cancer group) using differentially expressed gene (DEG) analysis. The RNAs with both |log2FC| >2 and P values < 0.05 2. We prepared for log2 fold change values of plasma exosomal RNAs for 30 patients from 30 samples in second week during CCRT compared with those before treatment to screen secondary biological function of primarily selected DEGs. We analyzed the association between non-coding RNA (ncRNA)-mRNA network and cancer using ingenuity pathway analysis after secondary selection according to the number and correlation of mRNAs (or ncRNAs) relevant to log2FC in the expression of primarily selected ncRNAs (or mRNAs) before and after CCRT. The raw NGS data of 58 samples from 29 patients were uploaded in E-MTAB-10215. Here we will upload raw RNA sequencing data of 12 samples from 12 healthy individuals and 2 samples from one cervical cancer patient.
Project description:Genome wide DNA methylation profiling of cervical cancer samples and normal tissue. The Illumina Infinium 450K Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 450,000 CpGs in cervical cancer samples. Samples included 102 cervical cancer samples and 29 histologicall normal samples.
Project description:The role of non-coding RNAs in different biological processes and diseases is continuously expanding. Next-generation sequencing together with the parallel improvement of bioinformatics analyses allows the accurate detection and quantification of an increasing number of RNA species. With the aim of exploring new potential biomarkers for disease classification, a clear overview of the expression levels of common/unique small RNA species among different biospecimens is necessary. However, except for miRNAs in plasma, there are no substantial indications about the pattern of expression of various small RNAs in multiple specimens among healthy humans. Overall, the data reported hereby provide an insight of the constitution of the human miRNome and other small non-coding RNAs in various specimens of healthy individuals. This dataset was submitted by the Extracellular RNA Atlas (http://exrna-atlas.org/exat/datasets/EXR-ANACC1S6lJ1C-AN), and the selected raw and processed data for this dataset corresponds to what is available in that resource. Submitter indicates: "The publication associated with the citation below refers to a slightly larger set of samples (includes cervical scrape samples) and contains an alternative analysis to the processed data files provided here."
