Dataset Information
Heidelberg University Hospital, Cluster of Excellence CellNetworks, Center for Infectious Diseases, Virology, Heidelberg (BioQua
Ontology highlight
ABSTRACT:
PROVIDER: EGAO00000000576 | EGA |
REPOSITORIES: EGA
Publications
TALEN/CRISPR-mediated engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus.
Nucleic acids research 20160909 1
Successful RNAi applications depend on strategies allowing robust and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. Here, we propose a novel avenue which is integration of a promoterless shmiRNA, i.e. a shRNA embedded in a micro-RNA (miRNA) scaffold, into an engineered genomic miRNA locus. For proof-of-concept, we used TALE or CRISPR/Cas9 nucleases to site-specifically integrate an anti-hepatitis C virus (HCV) shmiRNA into the liver-speci ...[more]
