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Microinjection of hIPSC derived intestinal organoids with Salmonella Typhimurium

ABSTRACT: To generate an RNA-Seq dataset for organoids apically stimulated with Salmonella Typhimurium. These data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

PROVIDER: EGAS00001001253 | EGA |

REPOSITORIES: EGA

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Interaction of Salmonella enterica Serovar Typhimurium with Intestinal Organoids Derived from Human Induced Pluripotent Stem Cells.

Forbester Jessica L JL Goulding David D Vallier Ludovic L Hannan Nicholas N Hale Christine C Pickard Derek D Mukhopadhyay Subhankar S Dougan Gordon G

Infection and immunity 20150511 7

The intestinal mucosa forms the first line of defense against infections mediated by enteric pathogens such as salmonellae. Here we exploited intestinal "organoids" (iHOs) generated from human induced pluripotent stem cells (hIPSCs) to explore the interaction of Salmonella enterica serovar Typhimurium with iHOs. Imaging and RNA sequencing were used to analyze these interactions, and clear changes in transcriptional signatures were detected, including altered patterns of cytokine expression after ...[more]

PMID: 25964470

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