Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi

ABSTRACT: VPS15 is known as a VPS34-associated protein that functions in intracellular trafficking and autophagy. Here the authors identify a role for VPS15 in cilopathy and ciliary phenotypes, and show that it interacts with GM130 and functions in IFT20-dependent cis-Golgi to cilium trafficking.

PROVIDER: EGAS00001002075 | EGA |

REPOSITORIES: EGA

ACCESS DATA

Json Xml

Similar Datasets

New identification of novel proteins associated with xenophagy in HeLa cell

Project description:Almost all intracellular bacteria invade host cells through endocytic membrane trafficking. Bacteria that invade through endocytosis are rapidly transported to degradative organelles, therefore bacterial pathogens have evolved strategies to modulate intracellular bactericidal pathways during infection for survival and proliferation. On the other hand, the host cell also has a defensive system (xenophagy, a type of selective autophagy) against pathogens escaping from endosomes.

2023-05-23 | PXD042419 | JPOST Repository

Identification of novel proteins associated with xenophagy in host cell

2022-12-21 | PXD038868 | JPOST Repository

Cis-trans

Project description:Cis-trans

| PRJEB1227 | ENA

Cis boleti

Project description:Cis boleti overview

| PRJNA1053443 | ENA

Pervasive cis-antisense transcription negatively regulates gene expression in E. coli K12 in response to environmental changes

Project description:cis-antisense RNAs (cis-asRNAs) in prokaryotes are more pervasive than originally thought. However, little is known about their expression patterns and functions. Here we determined transcriptomes and proteomes of E. coli at multiple time points in five culture conditions using directional RNA-seq and tandem mass spectrometry. We found that a highly varying portion (27.2%~90.8%) of transcribed genes had cis-asRNAs dependent on growth phases and culture conditions, and that almost all transcribed genes changed their cis-asRNA levels relative to the mRNA level at different growth phases and culture conditions. Intriguingly, the correlation between the protein and mRNA levels of genes is abolished the increasing cis-asRNA levels relative to the mRNA levels, suggesting that cis-asRNAs might directly or indirectly inhibit translation by forming duplexes with mRNAs. All data sets are available for data mining from a unified resource to support further biological discoveries and insights into cis-asRNA-mediated gene expressional regulation.

2022-02-28 | PXD001640 | Pride

CIS deficiency intensifies GM-CSF signaling to polarize alternative activated macrophages

Project description:GM-CSF paradoxically possesses ability to differentiate both classically activated macrophages (MØs) with dominant proinflammatory function (M1-like MØs) and alternative activated MØs with strong immunosuppressive function (M2-like MØs). The intrinsic regulatory mechanism responsible for functional polarization of MØs under GM-CSF signalling remains elusive. Here we revealed that cytokine-inducible SH2-containing protein (CIS), induced by GM-CSF, is a key determinant in controlling MØ polarization. Compared to WT MØs, Cish-/- MØs gained characteristics of alternative activated MØs (M2 MØs), showing high expression of prototypic M2 markers Arginase 1, Tgm2 and YM-1; strong suppression of T cell proliferation; and low production of IL-12 and other proinflammatory cytokines¬¬. Differing from canonical IL-4/STAT6/IRF4 signaling axis of M2 induction, development of M2 MØ characteristics in Cish-/- MØs was associated with intensified STAT5 activation and consequent IRF8 downregulation. Attenuation of GM-CSF signalling via JAK inhibition and IRF8 rescue corrected certain functional defects in Cish-/- MØs. As CIS inhibition in NK and T cells promotes anti-tumour immunity, we showed that CIS deficiency enhanced the development of intra-tumoural M2 MØs and reduced CTL induction within tumour microenvironment with elevated GM-CSF. Overall, we conclude that CIS acts as an intrinsic rheostat to control intense GM-CSF signalling in order to maintain proinflammatory functions of MØs. Targeting CIS as a checkpoint in cancer immunotherapy should consider its role in regulating myeloid cell function.

2023-01-21 | PXD018390 | Pride

CD137-CIS IO

Project description:CD137-CIS IO

| PRJNA663842 | ENA

cis-Expression QTL Analysis of Established Risk Variants for Colorectal Cancer

Project description:Genome-wide association studies (GWAS) have identified 19 risk variants associated with colorectal cancer. As most of these risk variants reside outside the coding regions of genes, we conducted cis-expression quantitative trait loci (cis-eQTL) analyses to investigate possible regulatory functions on the expression of neighboring genes. Forty microsatellite stable and CpG island methylator phenotype-negative colorectal tumors and paired adjacent-normal colon tissues were used for genome-wide SNP and gene expression profiling in our cis-eQTL analyses. This submission represents transcriptome component of study.

2012-01-31 | E-GEOD-31737 | biostudies-arrayexpress

CoV 3'UTR cis-acting element interactome

Project description:Comparison of CoV 3'UTR cis-acting element interactome to link the cis-acting element to coronavirus replication by LC-MS/MS. The study is performed by in vitro-transcribed RNA followed by RNA-protein pull-down assay. In addition, the concluded results are decided by comparison between the biological processes derived from analysis of interactome and the replication efficiency.

2024-08-07 | MSV000095535 | MassIVE

CoV 3'UTR cis-acting element interactome

2024-08-06 | MSV000095522 | MassIVE