Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole genome sequencing of in vitro colonies

ABSTRACT: In this study we will perform whole genome sequencing on in vitro colonies. Colonies of various sizes (100-300 cells) will be collected for somatic mutation analysis.

PROVIDER: EGAS00001003112 | EGA |

REPOSITORIES: EGA

ACCESS DATA

Json Xml

Similar Datasets

Targeted sequencing of in vitro colonies bulks

Project description:In this study we will perform targeted sequencing on the bulk samples of in vitro colonies

| EGAS00001003175 | EGA

colonies

Project description:colonies Metagenome

| PRJNA673756 | ENA

RNA sequencing of Pseudomonas aeruginosa phoenix colonies, viable but non-culturable colonies (VBNCs), and control samples

Project description:We report RNA sequencing data for mRNA transcripts obtained from tobramycin exposed phoenix colonies, VBNCs, and various controls (untreated lawn, edge of the zone of clearance of tobramycin, treated outer background lawn). Extracted mRNA was sequenced using an Illumina HiSeq 4000, mapped to a Pseudomonas aeruginosa PAO1 reference genome, and processed to obtain counts for all gene transcripts for each sample. This is the first sequencing data generated for Pseudomonas aeruginosa phoenix colonies and VBNCs.

2022-03-27 | GSE199323 | GEO

Comparison of gene expression profiles of E. coli W3110 normal colonies vs. L-form colonies

Project description:Expression data comparing typical E.coli colonies grown on BHI and BHI+Sucrose+MgSO4 to L-form colonies grown on BHI+Sucrose+MgSO4+Penicillin G. Keywords: one time point

2010-02-01 | GSE14796 | GEO

Hybridisation of 11 8-cell neonatal foreskin keratinocyte colonies

Project description:Single stem cells, including those in human epidermis, have a remarkable ability to reconstitute tissues in vitro, but the cellular mechanisms which enable this are ill defined. Using live imaging we saw two populations of cells those which generate expanding colonies and those which generate balanced colonies. We wanted to assess if there were any transcriptional differences between these two colony types.

2015-10-19 | E-MTAB-3128 | biostudies-arrayexpress

Comparison of gene expression profiles of E. coli W3110 normal colonies vs. L-form colonies

Project description:Expression data comparing typical E.coli colonies grown on BHI and BHI+Sucrose+MgSO4 to L-form colonies grown on BHI+Sucrose+MgSO4+Penicillin G. Keywords: one time point DNA microarray analysis of E.coli colonies grown on BHI and BHI+Sucrose+MgSO4 (controls) with L-form colonies grown on BHI+Sucrose+MgSO4+Penicillin G (experimental). An isolated colony of fresh W3110 was grown for 5hrs to log phase, dilutions of the inoculmn were spread onto three media: BHI, BHI+Sucrose+MgSO4, and BHI+Sucrose+MgSO4+Penicillin G to yield isolated colonies. Isolated control colonies which grow overnight were harvested the next day while isolated L-form colonies which do not grow until 48-72 hrs were harvested after 72hrs. All colonies were harvest with a PBS moistened sterile swab and dipped into a sterile microcentrifuge tube containing 500ul of PBS. RNA was isolated from pelleted cells using MasterPure RNA Purification Kit and reverse transcribed for making probes for array hybridization on E.coli Genome 2.0 Affymetrix microarrays.

2010-02-21 | E-GEOD-14796 | biostudies-arrayexpress

Transcriptional profiling of 2D colonies derived from WT, Bmi1-/-, and Bmi1-/-;Ink4a/Arf Labial cervical loops

Project description:This experiment is aimed at determining the genetic signature of dental stem cell colonies grown in vitro and how that signature changes when Bmi1 activity is removed. Another question to be answered by the experiment is whether Bmi1 has alternate targets besides Ink4a/Arf. There are 12 total samples: 4 biological replicates for each genotype that consist of single colonies grown in vitro.

2013-04-12 | E-GEOD-46000 | biostudies-arrayexpress

EGAS00001003112-sc-2020-05-19T13:18:08Z - samples

Project description:In this study we will perform whole genome sequencing on in vitro colonies.

| EGAD00001006162 | EGA

Colonies from Waste Sewage Sludge

Project description:Colonies from Waste Sewage Sludge Metagenome

| PRJNA670592 | ENA

sok2 vs wild type colonies

Project description:Time course analyses of gene expression in sok2 deleted vs wild type colonies during late developmental stages on plate. Keywords = ammonia Keywords = metabolism Keywords = yeast Keywords = stress Keywords = transcriptome Keywords: time-course

2004-07-11 | GSE1454 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data