ABSTRACT: This study aims to conduct a landscape analysis of transcriptome from a patient with CHD8 deletion. RNAseq was conducted on a patient and normal sibling, each sample in triplicate.
Project description:To define the hedgehog-interacting protein (Hhip) expression domain in adult mouse lung, we conduct single-cell RNAseq of the mouse lung using 10X scRNAseq technique.
Project description:Zebrafish primary neurons either sfpq sibling/control (+/+, +/-) or null (-/-) were cultured in transwell inserts. At DIV2, for each genotype, cellular and neurite tissues were separately isolated, RNA extracted and total RNAseq performed.
Project description:To study resident immune cells in Hhip-deleted lungs, we conduct single-cell RNAseq of the resident immune cells from Gli1HhipCKO_Tam and Gli1HhipCKO_Oil_Control mice lungs using 10X genomics scRNAseq technique.
Project description:To dissected the roles of SMAD2/3 and SMAD4 in mouse embryonic stem cells (mESC), knockouts of SMAD2/3 (SMAD2 and SMAD3 double knockout, S2/3DKO) and SMAD4 (SMAD4 knockout, S4KO) were used to conduct RNAseq in ES and EB conditions.
Project description:Identification of the difference in transcriptome between wild-type and SEC66 deletion mutant cells poly-A stranded RNAseq of three isolates of wild-type and three isolates of sec66â?? cells
Project description:The transcriptome of zebrafish with morphologically mutant phenotypes associated with knockout alleles were analyised using Illumina sequencing to detect differential transcript abundance. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph7 Total RNA was extracted from morpholically abnormal and sibling wild type embryos from 3 clutches using Trizol. After DNase treatment non-directional RNAseq libraries were made using the TrueSeq protocol with sample indexing. Libraries were 75 base paired-end sequenced by Illumina HiSeq. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:Long-term hematopoietic stem cells (HSCs), short-term HSCs and multipotent progenitor cells (MPPs) were isolated from bone marrow of four mouse strains (WT, H19-deletion, Igf1r-deletion, and double-deletion) and expression profiled with RNAseq. The behavior of the transcriptomes, and in particular the imprinted genes, was analyzed to see what might be involved in maintaining quiescence of long-term stem cells, and how H19 and Igf1r affected the expression of imprinted genes. Transcriptional profiling data of the same cells have been deposited in ArrayExpress under accession number E-MTAB-1644 (http://wwwdev.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1644/).
