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ScRNA-seq of patient-derived PDAC organoids

ABSTRACT: We derived PDAC organoids from primary tumors of 18 patients, together with two matched samples from liver metastases. By single-cell RNA sequencing, we show that PDAC organoids consist of ductal cells with patient-specific expression of several gene groups, including genes which encode cell surface proteins. We report Ã¢Â€Â˜classicalÃ¢Â€Â™ and Ã¢Â€Â˜basal-likeÃ¢Â€Â™ cells coexisting within single primary tumors or metastases, with greater intratumor subtype heterogeneity linked to higher tumor grade. Single-cell transcriptome analysis of PDAC organoids and primary PDAC identified distinct tumor cell states shared across patients, including a cycling progenitor cell state and a differentiated secretory state. We show that these cell states are connected by a differentiation hierarchy, with Ã¢Â€Â˜classicalÃ¢Â€Â™ subtype cells concentrated at the endpoint of this hierarchy.

PROVIDER: EGAS00001004661 | EGA |

REPOSITORIES: EGA

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