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Quantitative and dynamic catalogs of proteins released during apoptotic and necroptotic cell death

ABSTRACT: The inflammatory functions of the cytokine tumor necrosis factor (TNF) rely on its ability to induce cytokine production and to induce cell death. Caspase dependent and independent pathways Ã¢â‚¬â€œ apoptosis and necroptosis Ã¢â‚¬â€œ respectively, regulate immunogenicity by the release of distinct sets of cellular proteins. To obtain an unbiased, systems-level understanding of this important process, we here applied mass spectrometry-based proteomics to dissect protein release during apoptosis and necroptosis. We report hundreds of proteins released from human myeloid cells in time-course experiments. Both cell death types induce receptor shedding, but only apoptotic cells released nucleosome components. Conversely, necroptotic cells release lysosomal components by activating lysosomal exocytosis at early stages of necroptosis- induced membrane permeabilisation and show reduced release of conventionally secreted cytokines.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Culture

SUBMITTER: Mario Oroshi

LAB HEAD: Felix Meissner

PROVIDER: PXD014966 | Pride | 2020-07-18

REPOSITORIES: Pride

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			Action	DRS
	20170725_QEp8_MaTa_QC_LC12_DMSO_1h_3.raw	Raw
	20170725_QEp8_MaTa_QC_LC12_DMSO_1h_4.raw	Raw
	20170725_QEp8_MaTa_QC_LC12_DMSO_3h_2.raw	Raw
	20170725_QEp8_MaTa_QC_LC12_DMSO_3h_3.raw	Raw
	20170725_QEp8_MaTa_QC_LC12_DMSO_3h_4.raw	Raw

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Quantitative and dynamic catalogs of proteins released during apoptotic and necroptotic cell death

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