Dataset Information

Plant health identification of Coleoptera

ABSTRACT: This project aims to establish new diagnostic methods for potentially invasive beetle species, which can occur within plant health controls

INSTRUMENT(S): Illumina NextSeq 500

ORGANISM(S): Anoplophora Glabripennis

SUBMITTER: University of Hohenheim

PROVIDER: PRJEB66443 | EVA | 2023-10-01

REPOSITORIES: EVA

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Dataset's files

Source:

			Action	DRS
	Variants_raw_185Indv_A-Z.fixed.accessioned.vcf.gz	Vcf
	Variants_raw_185Indv_A-Z.fixed.accessioned.vcf.gz.csi	Vcf
	Variants_raw_185Indv_A-Z.fixed.vcf.csi	Other
	Variants_raw_185Indv_A-Z.fixed.vcf.gz	Vcf

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Project description:Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. Due to particular physiological characteristics, no treatments against diseases caused by oomycetes are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phytophthora species and host plants. The present project is focused on the molecular mechanisms that underlie the compatible plant-oomycete interaction and plant disease.The laboratory developed a novel interaction system involving the model plant, Arabidopsis thaliana and Phytophthora parasitica, a soil-borne pathogen infecting a wide host range, thus representing the majority of Phytophthora species. A characteristic feature of the compatible Arabidopsis/Phytophthora parasitica interaction is an extended biotrophic phase, before infection becomes necrotrophic. Because the initial biotrophic phase is extremely short on natural (e.g. solanaceous) hosts, the Arabidopsis system provides the opportunity to analyze, for both interaction partners, the molecular events that determine the initiation of infection and the switch to necrotrophy.The present project aims at analyzing the compatible interaction between A. thaliana roots and Phytophthora parasitica. The Affymetrix A. thaliana full genome chip will be used to characterize modulations of the transcriptome occurring over a period of 24h from the onset of plant root infection to the beginning of necrotrophy. Parallel to this study, a custom designed Phytophthora parasitica biochip will enable analyzing of Phytophthora parasitica gene expression during the same stages. The pathosystem involving A. thaliana and Phytophthora parasitica was described in Attard A, Gourgues M, Callemeyn-Torre N, Keller H. 2010. The New phytologist 187: 449–460. The protocol for recovery of RNA from purified appressoria was described in Kebdani N, Pieuchot L, Deleury E, Panabieres F, Le Berre JY, Gourgues M. 2010. New Phytol 185: 248–257.

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Plant health identification of Coleoptera

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