0-12 hour transcriptional response of mouse bone marrow-derived macrophages to stimulation by LPS

ABSTRACT: Innate immune response to pathogens is a complex, multi-staged process involving thousands of genes. While numerous transcription factors that act as master regulators of the response to LPS have been identified, the temporal complexity of gene expression changes strongly suggest that additional layers of regulation remain to be uncovered. This study was done to examine response of mouse bone marrow-derived macrophages to stimulation with LPS over time. Bone marrow-derived macrophages from C57BL/6J mice were incubated in the presence of LPS for 1, 4 or 12 h, while the control group was incubated under same conditions for 4 h in the absence of the LPS. RNA isolated from these macrophages was analyzed using the Affymetrix Mouse Exon Array 1.0 ST.

ORGANISM(S): Mus musculus

PROVIDER: GSE100059 | GEO | 2017/06/16

SECONDARY ACCESSION(S): PRJNA390608

REPOSITORIES: GEO