Project description:T cells from spleen and lymphnode were isolated with EasySep Mouse CD8+ T Cell Isolation kit. Isolated CD8+ T cells were stimulated and cultured with plate-bound anti-CD3 and soluble anti CD28 antiboies in Tc1 or Tc9 cell polarizing conditios. After 3-day of polarization, cell were transferred into a new well and culture for another 2-day. on day 5 after seeding, Tc1 and Tc9 cells were collected and sequenced. We used microarrays to detail the global programme of gene expression of Tc1 and Tc9 cells at day 5 polarization.
Project description:we use RNA sequence to detect the global gene expression in tumor-infiltrating Tc1 and Tc9 cells after being adoptively transferred in vivo
Project description:The goal of this project is to dissect the mechanism by which Tc1 and Tc2 effector subtypes effect cell physiology in vitro. I utilized cellular proteomics to determine Tc1 and Tc2 secretion patterns and identify inflammatory factors that regulate cell polarization and physiology in vitro.
