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Identification of quiescence and early activation genes in satellite cells

ABSTRACT: We report the transcriptional changes that occur during the isolation procedure of adult skeletal muscle stem cells (MuSCs). We have developed a protocol that is based on the in situ fixation of MuSCs before isolation. The transcriptome of the in situ fixed cells was compared to that of MuSCs isolated using the standard mechanical/enzymatic isolation protocol. The differentially expressed transcripts represent early-response genes, involved in quiescence and activation of MuSCs, but also isolation-induced artefacts.

ORGANISM(S): Mus musculus

PROVIDER: GSE101576 | GEO | 2017/11/13

SECONDARY ACCESSION(S): PRJNA394867

REPOSITORIES: GEO

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Identification of quiescence and early activation histones modifications pattern in satellite cells

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