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Synthetic lethal siRNA screen for TN-IBC cell line SUM149PT using kinome library with EPA treatment

ABSTRACT: Synthetic lethal siRNA screen using a kinome library containing 939 druggable genes in the TN-IBC cell line SUM149PT, in combination with EPA treatment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE102057 | GEO | 2019/01/31

REPOSITORIES: GEO

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Homo sapiens

Project description:Synthetic lethal siRNA screen for TN-IBC cell line SUM149PT using kinome library with EPA treatment

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Project description:Purpose: Transcriptome profiling (RNA-seq) of a novel human Inflammatory Breast Cancer cell line A3250 in comparison to SUM149 and MDA-MB-231 Inflammatory Breast Cancer (IBC) is the most aggressive form of breast cancer with distinct clinical and histopathological features, but understanding of the unique aspects of IBC biology lags far behind that of other breast cancers. We describe a novel triple-negative IBC cell line, A3250, that recapitulates key features of human IBC in a mouse xenograft model.The purpose of this study was to compare differences in gene expression between A3250 IBC, MDA-MB-231 non-IBC and SUM149 IBC that does not present with typical clinical sympotms of IBC in a mouse model, with the goal of identifying unique molecular features for this unique type of breast cancer Results: RNA-Seq analysis identified expression profile characteristic for the novel A3250 IBC cell line, compared to SUM149 IBC and MDA-MB-231 non-IBC.

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EXoO-Tn: Tag-n-Map the Tn Antigen in the Human Proteome

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