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Gene expression profiling during active Lyme arthritis development (22 days post infection with B. burgdorferi) in CD45 negative cells isolated from joint tissue of highly genetically similar mouse strains: B6, ISRCL4, and ISRCL3

ABSTRACT: Previously, using a forward genetic approach we identified B. burgdorferi arthritis-associated locus 1 (Bbaa1), a quantitative trait locus on Chr4, which physically encompasses the type I IFN gene cluster and regulates Lyme arthritis through heightened type I IFN production. Reciprocal radiation chimeras between B6.C3-Bbaa1 and B6 mice revealed that arthritis is initiated by radiation-sensitive cells, but orchestrated by radiation-resistant components of joint tissue. Advanced congenic lines were developed to reduce the physical size of the Bbaa1 interval, and RNA-seq of resident CD45- joint cells from advanced interval specific recombinant congenic lines (ISRCL4 and ISRCL3) identified myostatin as uniquely upregulated in association with Bbaa1 arthritis development. Our manuscript further demonstrates that myostatin expression is linked to IFN-β production, and in vivo inhibition of myostatin suppresses Lyme arthritis in the reduced interval Bbaa1 congenic mice, formally implicating myostatin as a novel downstream mediator of joint-specific inflammatory response to B. burgdorferi.

ORGANISM(S): Mus musculus

PROVIDER: GSE102748 | GEO | 2018/02/13

REPOSITORIES: GEO

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The Cdkn2a gene product ARF is a critical regulator of IFNb mediated Lyme arthritis

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Project description:Gene expression profile of joint tissue from C3H and interval specific congenic mouse lines (ISCL) following infection with Borrelia burgdorferi Congenic lines of mice generated between C3H and C57BL/6 with penetrant arthritis severity phenotype were infected with B. burgdorferi Joint tissue was collected from uninfected control mice and from mice infected for 1 week, RNA was prepared and gene expressed analyzed by Affymetrix microarray.

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Transcription profiling of mouse joint tissue from C3H and interval specific congenic mouse lines post- B. burgdorferi infection

Project description:Gene expression profile of joint tissue from C3H and interval specific congenic mouse lines (ISCL) following infection with Borrelia burgdorferi; Congenic lines of mice generated between C3H and C57BL/6 with penetrant arthritis severity phenotype were infected with B. burgdorferi; Joint tissue was collected from uninfected control mice and from mice infected for 1 week, RNA was prepared and gene expressed analyzed by Affymetrix microarray. Experiment Overall Design: Each sample was comprised of pooled RNA from at least 5 female mice of each genotype.

2009-07-01 | E-GEOD-16195 | biostudies-arrayexpress

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2008-06-14 | E-GEOD-6055 | biostudies-arrayexpress

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2008-06-14 | E-GEOD-6092 | biostudies-arrayexpress

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2016-03-25 | E-MTAB-3325 | biostudies-arrayexpress

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2007-01-05 | GSE6092 | GEO

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