Effects of blackcurrant extract on gene expression on TIG113.
Ontology highlight
ABSTRACT: To investigate new health functions by blackcurrant extract (BCE), we compare altered of the genes expression before and after BCE (1.0μg/ml) treatment in TIG113.
Project description:To investigate new health functions by blackcurrant extract (BCE), we compare altered of the genes expression before and after BCE (1.0μg/ml) treatment in human follicle dermal papilla cells (HFDPC).
Project description:To investigate new health functions by blackcurrant extract (BCE), we compare altered of the genes expression before and after BCE (2.0μg/ml) treatment in Human Umbilical Vein Endothelial Cells (HUVEC).
Project description:To investigate new health functions by blackcurrant extract (BCE), we compare altered of the genes expression before and after BCE (50μg/ml) treatment in MCF10A.
Project description:To investigate new health functions by blackcurrant extract (BCE), we compare altered of the genes expression before and after BCE (50μg/ml) treatment in MCF10A. MCF10A cells were seeded in culture dish and maintain to confluent. Then medium replace with mammary epithelial cell growth medium kit with or without BCE (50μg/ml). After the cells were incubated for 24 h at 37 °C 5% CO2.
Project description:Blackcurrants (Ribes nigrum L., Grossulariaceae) have a high content of anthocyanin polyphenols and these have been shown to have beneficial effects on health, owing to their antioxidant and anti-carcinogenic properties. This study analyzed the constituents of blackcurrant extract (BCE) and investigated its potential phytoestrogenic effects using a breast cancer cell line (MCF-7) overexpressing the estrogen receptor (ER) α. Microarray and ingenuity pathway analysis showed that BCE activated upstream genes such as ERα and transforming growth factor beta 1, and upregulated the expression of many genes downstream of ERα. MCF-7 cells were seeded in culture dish and maintain to confluent. Then medium replace with phenol-red-serum-free DMEM medium with or without BCE (50μg/ml). After the cells were incubated for 24 h at 37 °C 5% CO2.
Project description:Blackcurrants (Ribes nigrum L., Grossulariaceae) have a high content of anthocyanin polyphenols and these have been shown to have beneficial effects on health, owing to their antioxidant and anti-carcinogenic properties. This study analyzed the constituents of blackcurrant extract (BCE) and investigated its potential phytoestrogenic effects using a breast cancer cell line (MCF-7) overexpressing the estrogen receptor (ER) α. Microarray and ingenuity pathway analysis showed that BCE activated upstream genes such as ERα and transforming growth factor beta 1, and upregulated the expression of many genes downstream of ERα.
Project description:To investigate the effects of transgenic lines L6 and L7 tomato fruits on total expression profile of MCF-7 breast cancer cells, we treated MCF-7 cells with 1 ug/ml of tomato fruit extract for 24 hours and compare it with wild type tomato fruit extract Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in MCF-7 cells treated with transgenic lines L6 and L7 tomatofruit extract and compare it to wild type tomato fruit extract.