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Gene Expression Profiling Provides Insights into the Moleculor Mechanism of FGFR2+ Fibrocyte-to-Cancer Associated Fibroblast Differentiation in Esophageal Squamous Cell Carcinoma

ABSTRACT: To obtain an accurate overview of changes occurring during fibrocyte-to-cancer associated fibroblast differentiation in human esophageal squamous cell carcinoma (ESCC), we isolated circulating FGFR2+ fibrocytes and their paired FGFR2+ cancer associated fibroblasts (CAFs) from ESCC patients and compared their gene expression profiles by high-throughput RNA Sequencing. On average, about 55.08 million reads were obtained after eliminating low quality reads and 52.76 million reads (95.79%) were aligned to the human mRNA reference sequences. Transcripts corresponding to a total of 1,016 and 409 genes were found to be upregulated or downregulated at least two-fold in the combined set of two CAF sample pools, respectively. Furthermore, we identified the potential master regulators of CAF-specific extracelluar factors by motif enrichment analysis and addressed the molecular mechanisms underlying CAF differentiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE103111 | GEO | 2020/08/01

REPOSITORIES: GEO

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