Project description:We aimed at analyzing the transcriptome changes associated with MIR205HG knock-down in RWPE-1 cells or overexpression in DU145 cells
Project description:We aimed at analyzing the transcriptome changes associated with the deletion of a portion of the Alu element from MIR205HG transcript
Project description:Aside serving as host gene for the microRNA miR-205, MIR205HG transcribes for a nuclear chromatin-associated long noncoding RNA (lncRNA) able to restrain the differentiation of prostate basal cells, a finding that led to its reannotation as LEADR (Long Epithelial Alu-interacting Differentiation-related RNA). In our previous work (PMID: 30659180), we showed that genes that are modulated upon manipulation of MIR205HG/LEADR are characterized by the presence of an Alu sequence in their promoters. Notably, an Alu element also spans the first and second exon of MIR205HG/LEADR, thus suggesting its possible involvement in target selection and binding. Here, we performed Chromatin Isolation by RNA precipitation followed by DNA-sequencing (ChIRP-seq) to map MIR205HG/LEADR chromatin occupancy at a genome-wide level in prostate basal cells.
Project description:RNA was isolated from mouse skin and pituitary. This was used for gene comparisons with an affymatrix Clariom S array We compared the differences in transcripts expressed in the skin and the pituitary in the presence/absence of MIR205HG- and miR-205, which revealed significant differences in the pituitary and overlap in the skin
