Project description:We introduce single-cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells drop casted onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enables a scalable approach to single-cell proteomics. This new scPiMS platform addresses the throughput bottleneck in single cell proteomics and boosts cell processing rate by several fold while accessing protein composition with higher coverage.
Project description:A cell suspension was prepared from wild-type P14 mouse retinas, and single-cell mRNAseq libraries were generated with Drop-Seq. Drop-Seq was performed on four separate days using the same age (P14) and strain (C57BL/6). On day 1, replicate 1 was obtained. On day 2, replicates 2 and 3 were obtained. On day 3, replicates 4-6 were obtained. On day 4, replicate 7 was obtained.
Project description:Cell suspensions were prepared from mouse lungs dissociated at baseline or 14 days after intratracheal bleomycin injury, and single cell mRNA-seq libraries were generated with Drop-seq.
Project description:A single cell suspension was generated from IDOL KO and Control mouse hypothalami, and single-cell mRNAseq libraries were generated with Drop-Seq.
Project description:A single cell suspension was generated from 24hr TBI and Sham mouse hippocampi, and single-cell mRNAseq libraries were generated with Drop-Seq.