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AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE104053 | GEO | 2019/06/14

REPOSITORIES: GEO

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AGO CLIP Reveals an Activated Network for Acute Regulation of Brain Glutamate Homeostasis in Ischemic Stroke.

Project description:Post-transcriptional regulation by microRNAs (miRNAs) is essential for complex molecular responses to physiological insult and disease. Although many disease-associated miRNAs are known, their global targets and culminating network effects on pathophysiology remain poorly understood. We applied Argonaute (AGO) crosslinking immunoprecipitation (CLIP) to systematically elucidate altered miRNA-target interactions in brain following ischemia and reperfusion (I/R) injury. Among 1,190 interactions identified, the most prominent was the cumulative loss of target regulation by miR-29 family members. Integration of translational and time-course RNA profiles revealed a dynamic mode of miR-29 target de-regulation, led by acute translational activation and a later increase in RNA levels, allowing rapid proteomic changes to take effect. These functional regulatory events rely on canonical and non-canonical miR-29 binding and engage glutamate reuptake signals, such as glial glutamate transporter (GLT-1), to control local glutamate levels. These results uncover a miRNA target network that acts acutely to maintain brain homeostasis after ischemic stroke.

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AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 7]

Project description:In vivo microRNA-target interactions from adult mouse cortex were identified with CLEAR-CLIP, a modified AGO HITS-CLIP approach that allows direct ligation of microRNA and target within purified, endogenous AGO-RNA complexes.

2019-06-14 | GSE104051 | GEO

AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 6]

Project description:Human motor cortex (brodmann’s area 9) tissues analyzed by Argonaute HITS-CLIP to determine human transcriptome-wide AGO binding pattern.

2019-06-14 | GSE104048 | GEO

AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 5]

Project description:Cortical cultures treated with OGD (oxygen glucose deprivation) in the presence of miR-29 expressing lenti-virus were analyzed by RNAseq to assess differences in miR-29 target gene expression.

2019-06-14 | GSE104047 | GEO

AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 4]

Project description:Adult mouse cortical tissues after MCAO or sham surgery were analyzed by ribosomal profiling to assess differences in ribosome associated mRNA fragments in I/R injury model of stroke.

2019-06-14 | GSE104043 | GEO

AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 3]

Project description:Adult mouse cortical tissues after MCAO or sham surgery were analyzed by small RNAseq at various time points after reperfusion to assess differences in miRNA abundance in I/R injury model of stroke.

2019-06-14 | GSE104037 | GEO

AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 2]

Project description:Adult mouse cortical tissues after MCAO or sham surgery were analyzed by RNAseq at various time points after reperfusion to assess differences in mRNA abundance in I/R injury model of stroke.

2019-06-14 | GSE104036 | GEO

AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 1]

Project description:Adult mouse cortical tissues after MCAO or sham surgery were analyzed by Argonaute HITS-CLIP to assess differences in transcriptome-wide AGO binding patterns in I/R injury model of stroke.

2019-06-14 | GSE104034 | GEO

AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke

Project description:AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke

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AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 3]

Project description:AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 3]

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