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AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 5]

ABSTRACT: Cortical cultures treated with OGD (oxygen glucose deprivation) in the presence of miR-29 expressing lenti-virus were analyzed by RNAseq to assess differences in miR-29 target gene expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE104047 | GEO | 2019/06/14

REPOSITORIES: GEO

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AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 7]

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Project description:Post-transcriptional regulation by microRNAs (miRNAs) is essential for complex molecular responses to physiological insult and disease. Although many disease-associated miRNAs are known, their global targets and culminating network effects on pathophysiology remain poorly understood. We applied Argonaute (AGO) crosslinking immunoprecipitation (CLIP) to systematically elucidate altered miRNA-target interactions in brain following ischemia and reperfusion (I/R) injury. Among 1,190 interactions identified, the most prominent was the cumulative loss of target regulation by miR-29 family members. Integration of translational and time-course RNA profiles revealed a dynamic mode of miR-29 target de-regulation, led by acute translational activation and a later increase in RNA levels, allowing rapid proteomic changes to take effect. These functional regulatory events rely on canonical and non-canonical miR-29 binding and engage glutamate reuptake signals, such as glial glutamate transporter (GLT-1), to control local glutamate levels. These results uncover a miRNA target network that acts acutely to maintain brain homeostasis after ischemic stroke.

| S-EPMC6784548 | biostudies-literature

AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke

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