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Measuring gene expression and RNA editing in Drosophila adapting to divergent microclimates

ABSTRACT: We performed RNA-seq to profile gene expression in the heads and whole bodies of 32 isofemale fly lines from two divergent microclimates at 'Evolution Canyon' in Israel (16 fly lines from each microclimate). We also measured RNA editing levels in the head tissue of these flies.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE104073 | GEO | 2017/09/20

SECONDARY ACCESSION(S): PRJNA408160

REPOSITORIES: GEO

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Measuring gene expression and RNA editing in Drosophila adapting to divergent microclimates

Project description:Measuring gene expression and RNA editing in Drosophila adapting to divergent microclimates

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Project description:Determining the mechanisms by which a species adapts to its environment is a key endeavor in the study of evolution. In particular, relatively little is known about how transcriptional processes are fine-tuned to adjust to different environmental conditions. Here we study Drosophila melanogaster from 'Evolution Canyon' in Israel, which consists of two opposing slopes with divergent microclimates. We identify several hundred differentially expressed genes and dozens of differentially edited sites between flies from each slope, correlate these changes with genetic differences, and use CRISPR mutagenesis to validate that an intronic SNP in prominin regulates its editing levels. We also demonstrate that while temperature affects editing levels at more sites than genetic differences, genetically regulated sites tend to be less affected by temperature. This work shows the extent to which gene expression and RNA editing differ between flies from different microclimates, and provides insights into the regulation responsible for these differences.

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Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates

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