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Determination of trastuzumab responsive microRNAs in SKBR3 and BT474 cells.

ABSTRACT: To develop gene-miRNA and pathway-miRNA networks in trastuzumab treatment, we performed a recent miRNA microarray profiling in trastuzumab treated/untreated SKBR3 and BT474 cell lines. The cells were plated at a starting density of 2 millions in 100 mm cell culture dishes and then, they were treated with 6 ug/mL trastuzumab and PBS in two replicates. Total RNA was isolated with the TRIzol reagent (Invitrogen) according to the manufacturer's instructions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE104076 | GEO | 2017/09/21

SECONDARY ACCESSION(S): PRJNA408159

REPOSITORIES: GEO

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