Genomics

Dataset Information

0

Targeted in situ genome-wide profiling with high efficiency for low cell numbers


ABSTRACT: Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an epigenomic profiling strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end DNA sequencing. As only the targeted fragments enter into solution, and the vast majority of DNA is left behind, CUT&RUN has exceptionally low background levels. CUT&RUN outperforms the most widely- used Chromatin Immunoprecipitation (ChIP) protocols in resolution, signal-to-noise, and depth of sequencing required. In contrast to ChIP, CUT&RUN is free of solubility and DNA accessibility artifacts and has been used to profile insoluble chromatin and to detect long-range 3D contacts without cross-linking. Here we present an improved CUT&RUN protocol that does not require isolation of nuclei and provides high-quality data starting with only 100 cells for a histone modification and 1000 cells for a transcription factor. From cells to purified DNA CUT&RUN requires less than a day at the lab bench and requires no special skills.

ORGANISM(S): Homo sapiens

PROVIDER: GSE104550 | GEO | 2018/03/21

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-07-15 | GSE145187 | GEO
2023-04-28 | GSE213150 | GEO
2021-02-08 | GSE153020 | GEO
2019-06-24 | GSE126612 | GEO
2024-06-20 | GSE253630 | GEO
2019-02-22 | GSE123602 | GEO
2022-07-15 | GSE193803 | GEO
2023-01-04 | GSE213467 | GEO
2024-06-20 | GSE253632 | GEO
2022-01-01 | GSE165417 | GEO