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Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [RNA-seq2]

ABSTRACT: Here we were performed RNAseq to analyze the transcriptome changing of Hoxb5 in Pro/pre-B by over expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE105768 | GEO | 2018/02/07

REPOSITORIES: GEO

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Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [ChIP-Seq]

Project description:Chipseq were performed to analyze the targets of Hoxb5 in pro-/pre-B by over expression.

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Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes.

Project description:Deletion of master regulators of the B cell lineage reprograms B cells into T cells. Here we found that the transcription factor Hoxb5, which is expressed in uncommitted hematopoietic progenitor cells but is not present in cells committed to the B cell or T cell lineage, was able to reprogram pro-pre-B cells into functional early T cell lineage progenitors. This reprogramming started in the bone marrow and was completed in the thymus and gave rise to T lymphocytes with transcriptomes, hierarchical differentiation, tissue distribution and immunological functions that closely resembled those of their natural counterparts. Hoxb5 repressed B cell 'master genes', activated regulators of T cells and regulated crucial chromatin modifiers in pro-pre-B cells and ultimately drove the B cell fate-to-T cell fate conversion. Our results provide a de novo paradigm for the generation of functional T cells through reprogramming in vivo.

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Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [RNA-Seq]

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Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes

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Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

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Hoxb5 reprograms murine multipotent blood progenitors into hematopoietic stem cell-like cells

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