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Peripheral whole blood mRNAs and lncRNAs expression analysis in eosinophilic asthmatics

ABSTRACT: Long non-coding RNA (lncRNA) plays roles in many diseases including asthma. Several lncRNAs function in the early differentiation of T-helper cells. lncRNA controls gene transcription, protein expression and epigenetic regulation; As one of the four asthma phenotypes, eosinophilic asthma(EA) occupies the largest proportion in asthma patients; However, lncRNA associated with eosinophilic asthma have not to be identified so far. We designed the study to identify the circulating lncRNA signature in EA samples. We tested whether any significant changes in lncRNA expression was observed in EA and healthy people (Control) sample’ blood samples. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed through lncRNA-mRNA co-expression network. lncRNA expression was measured by means of microarray, quantitative real-time PCR. A total of 41 dysregulated lncRNAs and 271 dysregulated mRNAs (difference ≥2-fold) were found in EA compared to Control samples. GO terms and KEGG pathway annotation data revealed that several lncRNA were significantly associated with EA. By qRT-PCR, lncRNA is confirmed significantly expression between EA and control samples. The results presented here show several lncRNA may take part in the immune process of EA. Whether these lncRNA can be used as biomarkers need to be further study in future trials

ORGANISM(S): Homo sapiens

PROVIDER: GSE106230 | GEO | 2019/04/09

REPOSITORIES: GEO

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