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CHRAC/ACF Contribute to the Repressive Ground State of Chromatin

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE106733 | GEO | 2018/02/05

REPOSITORIES: GEO

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CHRAC/ACF contribute to the repressive ground state of chromatin.

Project description:The chromatin remodeling complexes chromatin accessibility complex and ATP-utilizing chromatin assembly and remodeling factor (ACF) combine the ATPase ISWI with the signature subunit ACF1. These enzymes catalyze well-studied nucleosome sliding reactions in vitro, but how their actions affect physiological gene expression remains unclear. Here, we explored the influence of Drosophila melanogaster chromatin accessibility complex/ACF on transcription by using complementary gain- and loss-of-function approaches. Targeting ACF1 to multiple reporter genes inserted at many different genomic locations revealed a context-dependent inactivation of poorly transcribed reporters in repressive chromatin. Accordingly, single-embryo transcriptome analysis of an Acf knock-out allele showed that only lowly expressed genes are derepressed in the absence of ACF1. Finally, the nucleosome arrays in Acf-deficient chromatin show loss of physiological regularity, particularly in transcriptionally inactive domains. Taken together, our results highlight that ACF1-containing remodeling factors contribute to the establishment of an inactive ground state of the genome through chromatin organization.

| S-EPMC6238394 | biostudies-literature

CHRAC/ACF Contribute to the Repressive Ground State of Chromatin [RNA-seq]

Project description:The chromatin remodeling complexes CHRAC and ACF combine the ATPase ISWI with the signature subunit ACF1. These enzymes catalyze well-studied nucleosome sliding reactions in vitro, but how their actions affect physiological gene expression is unclear. Here we explored the influence of Drosophila CHRAC/ACF on transcription by complementary gain- and loss-of-function approaches. Targeting ACF1 to multiple reporter genes inserted at many different genomic locations revealed a context-dependent inactivation of poorly transcribed reporters in repressive chromatin. Accordingly, single-embryo transcriptome analysis of a Acf knock-out allele showed that only lowly expressed genes are de-repressed in the absence of ACF1. Finally, the nucleosome arrays in Acf-deficient chromatin show loss of physiological regularity, particularly in transcriptionally inactive domains. Taken together our results highlight that ACF1-containing remodeling factors contribute to the establishment of an inactive ground state of the genome through chromatin organization.

2018-02-05 | GSE106732 | GEO

CHRAC/ACF Contribute to the Repressive Ground State of Chromatin [Mnase-seq]

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RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-03-29 | E-GEOD-79655 | biostudies-arrayexpress

CHRAC/ACF Contribute to the Repressive Ground State of Chromatin

Project description:CHRAC/ACF Contribute to the Repressive Ground State of Chromatin

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Derivation of novel human ground state naM-CM-/ve pluripotent stem cells.

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Chromatin state dynamics during blood formation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-08-08 | E-GEOD-60103 | biostudies-arrayexpress

CHRAC/ACF Contribute to the Repressive Ground State of Chromatin [Mnase-seq]

Project description:CHRAC/ACF Contribute to the Repressive Ground State of Chromatin [Mnase-seq]

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CHRAC/ACF Contribute to the Repressive Ground State of Chromatin [RNA-seq]

Project description:CHRAC/ACF Contribute to the Repressive Ground State of Chromatin [RNA-seq]

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The translational landscape of ground state pluripotency

Project description:This SuperSeries is composed of the SubSeries listed below.

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