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Analysis of npc48 overexpressing plants-Analysis of npc48 overexpressing plants.

ABSTRACT: rs07-02_npc48 - analysis of npc48 overexpressing plants - Identification of potential targets of the non-protein codding RNA npc48. - We have lines overexpressing npc48 that shhowed a leaf morphological phenotype (serrated leaves). The proportion of lines havin gthe phenotype is around 20% of total transformants. We wanted to compare the transcriiptome of npc48-overexpressing lines with and without phenotype together with a transformation control for an additional control. Keywords: gene knock in (transgenic)

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE10679 | GEO | 2008/04/12

SECONDARY ACCESSION(S): PRJNA107663

REPOSITORIES: GEO

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Project description:Accumulating information indicates that plant disease resistance signaling pathway frequently interact with other pathways regulating developmental processes or abiotic stress responses. However, the molecular mechanisms of these types of crosstalk remain poorly understood in most cases. Here we report that OsWRKY13, an activator of rice resistance to both bacterial and fungal pathogens, functions as a convergent point of the crosstalk between pathogen-induced salicylate-dependent defense pathway and at least five other physiological pathways. Genome-wide analysis of the expression profiling of OsWRKY13-overexpressing lines showed that OsWRKY13 directly or indirectly regulates the expression of more than 500 genes, which are potentially involved in different physiological processes according to the classification of Gene Ontology database. Comparing the expression patterns of genes functioning in known pathways or cellular processes upon pathogen infection and the phenotypes between OsWRKY13-overexpressing and susceptible wild-type plants, we find that OsWRKY13 is also regulator of other physiological processes during pathogen infection. OsWRKY13-involved disease resistance pathway synergistically interacts with glutathione/glutaredoxin system and flavonoid biosynthesis pathway via OsWRKY13 to monitor redox homeostasis and may enhance the biosynthesis of antimicrobial flavonoid phytoalexins. Meanwhile, OsWRKY13-invloved disease resistance pathway antagonistically interacts with SNAC1-mediated abiotic-stress defense pathway, JA signaling pathway, and terpenoid metabolism pathway via OsWRKY13 to suppress salt and cold defense responses as well as may retard rice growth and development. Experiment Overall Design: To determine the genes directly or indirectly regulated by OsWRKY13 genome-widely, we performed the microarray analysis using the Affymetrix Genechip Rice Genome Array containing total 57381 probe sets. Two independent OsWRKY13-overexpressing rice lines, D11UM 1-1 and D11UM 7-2 (Qiu et al., 2007), and wild-type Mudanjiang 8, which was susceptible to Xoo and M. grisea, were used for the analyses.The experiment was repeated three times.

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Analysis of npc48 overexpressing plants-Analysis of npc48 overexpressing plants.

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